Article No
AFC-I7WAOP-0.1
Application | FC |
Article No | AFC-I7WAOP-0.1 |
Biosite Brand | Biosite Flow |
Country Availability | all |
Clone | AE1 |
Clone Type | monoclonal |
Concentration | 0.1 mg/ml |
Conjugation | PE |
Description | Anti-Acidic Cytokeratins PE |
Recommended Dilution | The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography. |
Supplier | Nordic Biosite |
Format | PE |
Immunogen | Human epidermal keratins |
Isotype | IgG1 |
Keywords | Cytoskeleton: Intermediate Filaments (Veterinary), Cytoskeleton: Intermediate Filaments (Rodent), Cytoskeleton: Intermediate Filaments (Human) |
Notes | Cytokeratins are a subfamily of intermediate filaments and are characterized by remarkable biochemical diversity. CThey are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families. |
Alias Names | Type I cytokeratins |
Previous Article No | AFC-D4D202, AFC-I7WAOP-0.1 |
Product Type | Antibodies Primary |
Protocol | Flow cytometry: Recommended dilution: 2-5 μg/ml. Intracellular staining. |
Purification | Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
Size | 0.1 mg |
Source / Host | mouse |
Species Reactivity | rabbit, cat/feline, rat, mouse, human, dog/canine, chicken, monkey |
Storage | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
Substrate / Buffer | Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Technical Specifications | Mouse monoclonal antibody AE1 recognizes acidic type cytokeratins (intracellular antigens), namely K10, 14, 15, 16, 19 (40-56 kDa). This antibody stains well the basal layer of epidermis and most epithelia. |
Product Page Updated | 2024-01-03T12:18:20.318Z |