Article No
AFC-CXWMHD-0.1
Rat IgG2a Isotype Control APC
Rat IgG2a Isotype Control APC
Antibodies Isotype Control
Specifications
| Application | FC |
| Article No | AFC-CXWMHD-0.1 |
| Biosite Brand | Biosite Flow |
| Country Availability | all |
| Clone | RTG2A1-1 |
| Clone Type | monoclonal |
| Concentration | 0.1 mg/ml |
| Conjugation | APC |
| Description | Rat IgG2a Isotype Control APC |
| Supplier | Nordic Biosite |
| Format | APC |
| Isotype | IgG2a |
| Previous Article No | AFC-CXWMHD-0.1 |
| Product Type | Antibodies Isotype Control |
| Protocol | Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 μg/ml of incubation mixture), change the conditions of your experiment to reduce the background. |
| Purification | Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
| Size | 0.1 mg |
| Source / Host | rat |
| Storage | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
| Substrate / Buffer | Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Technical Specifications | This rat IgG2a kappa monoclonal antibody (clone RTG2A1-1, also known as G2A-1-1 or KLH/G2A-1-1) detects specifically the hemocyanin of keyhole limpet and can be used as a negative control antibody in other species. |
| Product Page Updated | 2024-01-03T12:18:20.318Z |
