Article No
AFC-2YIVSE-100
Application | FC |
Article No | AFC-2YIVSE-100 |
Biosite Brand | Biosite Flow |
Country Availability | all |
Clone | PC10 |
Clone Type | monoclonal |
Conjugation | PE |
Description | Anti-PCNA PE |
Recommended Dilution | The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary. |
Supplier | Nordic Biosite |
Entrez Gene ID | 5111 |
Format | PE |
Immunogen | recombinant rat PCNA |
Isotype | IgG2a k |
Keywords | Transcription Regulation & Nuclear Proteins (Veterinary), Transcription Regulation & Nuclear Proteins (Rodent), Transcription Regulation & Nuclear Proteins (Human) |
Notes | PCNA (proliferating cell nuclear antigen) is the DNA polymerase delta auxiliary protein acting in homotrimeric form to increase the processivity of leading strand synthesis during DNA replication. PCNA is expressed in the nucleus of all proliferating cells. In response to DNA damage, it is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. PCNA is a useful marker of DNA synthesis, as its form not involved in DNA synthesis degradates in histological preparations in the presence of organic solvents. |
Alias Names | ATLD2 |
Previous Article No | AFC-BC2E5C |
Product Type | Antibodies Primary |
Protocol | Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. Intracellular staining. |
Purification | Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
Size | 100 tests |
Source / Host | mouse |
Species Reactivity | mouse, human, monkey, insect, chicken, rat |
Storage | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
Substrate / Buffer | Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Technical Specifications | The mouse monoclonal antibody PC10 (also known as 3F81) recognizes PCNA, a 36 kDa conserved nuclear protein serving as a cofactor for DNA synthesis. |
UniProt Number | P12004 |
Product Page Updated | 2024-01-03T12:18:20.318Z |