Article No
AFC-S6I420-0.1
Application | FC, IP, WB, IHC, ICC |
Article No | AFC-S6I420-0.1 |
Biosite Brand | Biosite Flow |
Country Availability | all |
Clone | M38 |
Clone Type | monoclonal |
Concentration | 1 mg/ml |
Conjugation | Unconjugated |
Description | Anti-Hu CD81 Purified Low Endotoxin |
Supplier | Nordic Biosite |
Entrez Gene ID | 975 |
Format | Purified Low Endotoxin |
Immunogen | MOLT-4 (human T-ALL cell line) |
Isotype | IgG1 |
Keywords | CD Antigens & MHC (Veterinary), CD and Related Antigens (Human) |
Notes | CD81 (TAPA-1), a member of the tetraspanin family, is expressed on virtually all nucleated cells, but above all on germinal center B cells. CD81 forms complexes with other tetraspanin proteins, integrins, coreceptors, MHC class I and II molecules, and influences adhesion, morphology, activation, proliferation and differentiation of B, T and other cells, e.g. in muscles CD81 promotes cell fusion and myotube maintenance. CD81 has been also identified as a receptor for the hepatitis C virus. |
Alias Names | S5.7, CVID6, TAPA1, TSPAN28 |
Previous Article No | AFC-B1A0FD, AFC-S6I420-0.1 |
Product Type | Antibodies Primary |
Protocol | Functional application: In human MOLT-4 T-cell line the antibody M38 inhibits syncytium formation induced by coculture with human T-cell leukemia virus type 1 (HTLV-1)-positive human T-cell lines. Flow cytometry: recommended dilution: 1 μg/ml.Western blotting: recommended dilution: 1-2 μg/ml; positive control: Jurkat cells, non-reducing conditions. |
Purification | Purified by protein-A affinity chromatography. |
Purity | > 95% (by SDS-PAGE) |
Size | 0.1 mg |
Source / Host | mouse |
Species Reactivity | rabbit, cat/feline, human |
Storage | Store at 2-8°C. Do not freeze. |
Substrate / Buffer | Phosphate buffered saline (PBS), pH 7.4 |
Technical Specifications | The antibody M38 reacts with an extracellular epitope of CD81, a 25 kDa member of the tetraspanin family, expressed on majority of cells. |
UniProt Number | P60033 |
Product Page Updated | 2024-01-03T12:18:20.318Z |