Article No
AFC-AJ9MYR-100
Anti-Hu CD117 PE-Cy™7
Anti-Hu CD117 PE-Cy™7
Antibodies Primary
Specifications
| Application | FC |
| Article No | AFC-AJ9MYR-100 |
| Biosite Brand | Biosite Flow |
| Country Availability | all |
| Clone | 104D2 |
| Clone Type | monoclonal |
| Conjugation | PE/Cy7 |
| Description | Anti-Hu CD117 PE-Cy™7 |
| Supplier | Nordic Biosite |
| Entrez Gene ID | 3815 |
| Format | PE-Cy™7 |
| Immunogen | MOLM-1 megakaryocytic cells |
| Isotype | IgG1 k |
| Notes | CD117 / c-Kit (stem cell factor receptor) is a 145 kDa receptor tyrosine kinase that regulates cell proliferation, adhesion, chemotaxis, apoptosis and other cell processes. Mutations of CD117 / c-Kit can lead to growth and progression of tumours. After binding of its ligand, SCF (stem cell factor), CD117 / c-Kit is autophosphorylated on its intracellular domains and activated. CD117 is expressed on pluripotent hematopoietic progenitor cells, mast cells and various cancer cells, e.g. acute myeloid leukemia cells. |
| Alias Names | c-Kit, PBT, SCFR |
| Product Type | Antibodies Primary |
| Protocol | Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. |
| Purification | Purified antibody is conjugated with activated tandem dye of R-phycoerythrin-cyanine 7 (PE-Cy™7) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
| Size | 100 tests |
| Source / Host | mouse |
| Species Reactivity | monkey, human, cow/bovine |
| Storage | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
| Substrate / Buffer | Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Technical Specifications | The mouse monoclonal antibody 104D2 detects extracellular part of CD117 / c-Kit protooncogen. |
| UniProt Number | P10721 |
| Product Page Updated | 2024-01-03T12:18:20.318Z |
