Article No
AFC-LSA7ZU-0.1
Application | FC, IP |
Article No | AFC-LSA7ZU-0.1 |
Biosite Brand | Biosite Flow |
Country Availability | all |
Clone | CBRM1/5 |
Clone Type | monoclonal |
Concentration | 1 mg/ml |
Conjugation | Unconjugated |
Description | Anti-Hu CD11b (activation epitope) Purified Low Endotoxin |
Supplier | Nordic Biosite |
Entrez Gene ID | 3684 |
Format | Purified Low Endotoxin |
Immunogen | Information not available |
Isotype | IgG1 k |
Keywords | CD and Related Antigens (Human) |
Notes | CD11b (integrin alphaM subunit) is a 165-170 kDa type I transmembrane glycoprotein that non-covalently associates with integrin beta2 subunit (CD18); expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages. CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component. |
Alias Names | Mac-1, Integrin alpha M, ITGAM, CR3A, MO1A, MAC1A |
Previous Article No | AFC-A2F5C2, AFC-LSA7ZU-0.1 |
Product Type | Antibodies Primary |
Protocol | Functional application: Blocking. Flow cytometry: Recommended dilution: 1-4 µg/ml |
Purification | Purified by protein-A affinity chromatography. |
Purity | > 95% (by SDS-PAGE) |
Size | 0.1 mg |
Source / Host | mouse |
Species Reactivity | human |
Storage | Store at 2-8°C. Do not freeze. |
Substrate / Buffer | Phosphate buffered saline (PBS), pH 7.4 |
Technical Specifications | The mouse monoclonal antibody CBRM1/5 recognizes an activation-dependent epitope on extracellular part of CD11b (Mac-1alpha), a 165-170 kDa type 1 transmembrane protein mainly expressed on monocytes, granulocytes and NK-cells. The antibody recognizes a subset of CD11b molecules on neutrophils and monocytes activated with chemoattractants or phorbol esters and it does not recognize CD11b on non-activated cells. |
UniProt Number | P11215 |
Product Page Updated | 2024-01-03T12:18:20.318Z |