Article No
AFC-9UDT0B-100
Application | FC |
Article No | AFC-9UDT0B-100 |
Biosite Brand | Biosite Flow |
Country Availability | all |
Clone | GHI/61 |
Clone Type | monoclonal |
Conjugation | PE/Cy7 |
Description | Anti-Hu CD163 PE-Cy™7 |
Supplier | Nordic Biosite |
Entrez Gene ID | 9332 |
Format | PE-Cy™7 |
Immunogen | Hairy cell leukemia cells |
Isotype | IgG1 k |
Notes | CD163, also known as M130, is a member of the scavenger receptor family, accounting for the clearance of hemoglobin-haptoglobin complexes during limited hemolysis, which protects the body, in particular the kidneys, against heme-mediated oxidative damages. It does not have measurable affinity for noncomplexed hemoglobin or haptoglobin. Immunomodulatory role of CD163 has been postulated. CD163 is expressed by cells of the monocyte-macrophage lineage and its extracellular part also circulates in plasma as a soluble protein, especially during sepsis and other conditions affecting macrophage activity, when its level may raise manyfold. |
Alias Names | MM130, SCARI1 |
Product Type | Antibodies Primary |
Protocol | Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. |
Purification | Purified antibody is conjugated with activated tandem dye of R-phycoerythrin-cyanine 7 (PE-Cy™7) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
Size | 100 tests |
Source / Host | mouse |
Species Reactivity | human |
Storage | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
Substrate / Buffer | Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Technical Specifications | The mouse monoclonal antibody GHI/61 recognizes an extracellular epitope CD163, an approximately 130 kDa high affinity scavenger receptor expressed mainly on monocytes and macrophages, which binds hemoglobin-haptoglobin complex. |
UniProt Number | Q86VB7 |
Product Page Updated | 2024-01-03T12:18:20.318Z |