Article No
AFC-6ZA24W-100
Application | FC |
Article No | AFC-6ZA24W-100 |
Biosite Brand | Biosite Flow |
Country Availability | all |
Clone | 4G7 |
Clone Type | monoclonal |
Conjugation | PE/DyLight 594 |
Description | Anti-Hu CD19 PE-DyLight® 594 |
Recommended Dilution | The purified antibody is conjugated with tandem dye PE-DyLight™ 594 (PE-DL594) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary. |
Supplier | Nordic Biosite |
Entrez Gene ID | 930 |
Format | PE-DyLight<sup>®</sup> 594 |
Immunogen | Human CCL (chronic lymphocytic leukemia) cells |
Isotype | IgG1 k |
Keywords | CD and Related Antigens (Human) |
Notes | CD19 is a transmembrane glycoprotein of Ig superfamily expressed by B cells from the time of heavy chain rearrangement until plasma cell differentiation. It forms a tetrameric complex with CD21 (complement receptor type 2), CD81 (TAPA-1) and Leu13. Together with BCR (B cell antigen receptor), this complex signals to decrease B cell treshold for activation by the antigen. Besides being signal-amplifying coreceptor for BCR, CD19 can also signal independently of BCR coligation and it turns out to be a central regulatory component upon which multiple signaling pathways converge. Mutation of the CD19 gene results in hypogammaglobulinemia, whereas CD19 overexpression causes B cell hyperactivity. |
Alias Names | B4, Leu-12, CVID3 |
Previous Article No | AFC-5614-2 |
Product Type | Antibodies Primary |
Protocol | Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. |
Purification | Purified antibody is conjugated with activated tandem dye of R-phycoerythrin-DyLight®594 (PE-DyLight®594) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
Research Area | Immunology |
Size | 100 tests |
Source / Host | mouse |
Species Reactivity | human |
Storage | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
Substrate / Buffer | Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Technical Specifications | The mouse monoclonal antibody 4G7 recognizes an extracellular epitope of human CD19. |
UniProt Number | P15391 |
Product Page Updated | 2024-01-03T12:18:20.318Z |