Article No
AFC-GH39QC-0.1
Application | FC, IHC |
Article No | AFC-GH39QC-0.1 |
Biosite Brand | Biosite Flow |
Country Availability | all |
Clone | 9F10 |
Clone Type | monoclonal |
Concentration | 1 mg/ml |
Conjugation | Unconjugated |
Description | Anti-Hu CD49d Purified |
Supplier | Nordic Biosite |
Entrez Gene ID | 3676 |
Format | Purified |
Isotype | IgG1 k |
Keywords | CD and Related Antigens (Human) |
Notes | CD49d / integrin alpha 4, unlike other alpha integrins, neither contains an I-domain, nor undergoes disulfide-linked cleavage. It associates with beta 7 chain to form alpha 4 / beta 7 integrin, and with beta 1 chain (CD29) to form VLA-4 integrin. These complexes are important for lymphocyte migration from circulation into tissue (binding VCAM-1) and homing of T cell subsets to Peyer´s patches (binding MadCAM-1), but VLA-4 is also target for invasive bacteria which contain invasin. CD49d is essential for differentiation and migration of hematopoietic stem cells by their adhesion to bone marrow stromal cells, and provides a costimulatory signal to TCR-CD3 complex by inducing phosphorylation of some focal adhesion proteins. |
Alias Names | ITGA4, VLA-4 alpha |
Previous Article No | AFC-56035D, AFC-GH39QC-0.1 |
Product Type | Antibodies Primary |
Protocol | Immunohistochemistry: Recommended dilution: 5-10 μg/ml. |
Purification | Purified by protein-A affinity chromatography. |
Purity | > 95% (by SDS-PAGE) |
Research Area | Immunology |
Size | 0.1 mg |
Source / Host | mouse |
Species Reactivity | sheep/ovine, cat/feline, horse/equine, dog/canine, cow/bovine, monkey, human |
Storage | Store at 2-8°C. Do not freeze. |
Substrate / Buffer | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Technical Specifications | The mouse monoclonal antibody 9F10 recognizes an extracellular epitope of CD49d (alpha 4 integrin), a 145-180 kDa type I transmembrane glycoprotein expressed on B and T cells, monocytes, eosinophils, basophils, NK cells, and dendritic cells, but not platelets. |
UniProt Number | P13612 |
Product Page Updated | 2024-01-03T12:18:20.318Z |