CD8

Article No AFC-5602-2
Product Type Antibodies Primary
Research Area Immunology
Application FC, IP
Size 100 tests

Specifications

Country Availability all
BioSite Brand BioSite Flow
Clone MEM-31
Clone Type monoclonal
Conjugation PE/DyLight 594
Description Mouse Monoclonal to CD8
Recommended Dilution The purified antibody is conjugated with tandem dye PE-DyLight™ 594 (PE-DL594) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
Supplier BioSite Flow
Immunogen Crude thymus membrane fraction.
Isotype IgG2a
Keywords CD and Related Antigens (Human)
Notes The CD8 T cell coreceptor (monomer approx. 32-34 kDa) is expressed as alpha/beta heterodimer on majority of MHC I-restricted conventional T cells and thymocytes and as alpha/alpha homodimer on subsets of memory T cells, intraepithelial lymphocytes, NK cells and dendritic cells. Regulation of CD8 beta level on T cell surface seems to be an important mechanism to control their effector function. Assembly of CD8 alpha-beta but not alpha-alpha dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling.
Species Reactivity human
Storage Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Substrate / Buffer The reagent is provided in stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide.
Technical Specifications The antibody MEM-31 recognizes a conformationally-dependent epitope of CD8, a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. CD8 is a disulfide-linked dimer and exists as a CD8 alpha/alpha homodimer or CD8 alpha/beta heterodimer (each monomer approx. 32-34 kDa).<br><b>The antibody does not react with formaldehyde-fixed cells; negative in Western Blotting application.</b><br><br><b>HLDA III; WS Code T 575</b>
Product Page Updated 2018-07-27T09:12:01.609Z

Shipping info

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Species reactivity:

human human

Suggested protocols:

Immunoprecipitation

Immunoprecipitation