Article No
AFC-N7WAUU-0.1
Application | FC |
Article No | AFC-N7WAUU-0.1 |
Biosite Brand | Biosite Flow |
Country Availability | all |
Clone | 145-2C11 |
Clone Type | monoclonal |
Concentration | 0.5 mg/ml |
Conjugation | PErCP |
Description | Anti-Ms CD3 PerCP |
Recommended Dilution | The purified antibody is conjugated with Peridinin-chlorophyll-protein complex (PerCP) under optimum conditions. The conjugate is purified by size-exclusion chromatography. |
Supplier | Nordic Biosite |
Entrez Gene ID | 12501 |
Format | PerCP |
Immunogen | Mouse BM10-37 cytotoxic T lymphocytes |
Isotype | IgG k |
Keywords | CD and Related Antigens & MHC (Rodent) |
Notes | CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkynje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. |
Alias Names | CD3E, T3E, TCRE |
Previous Article No | AFC-5544-2, AFC-N7WAUU-0.1 |
Product Type | Antibodies Primary |
Protocol | Flow cytometry: Recommended dilution: 2-5 μg/ml. |
Purification | Purified antibody is conjugated with activated Peridinin-Chlorophyll Protein (PerCP) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
Research area | Immunology |
Size | 0.1 mg |
Source / Host | mouse |
Species Reactivity | mouse |
Storage | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
Substrate / Buffer | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Technical Specifications | The Armenian hamster monoclonal antibody 145-2C11 reacts with an extracellular epitope of murine CD3 (epsilon subunit). This antibody is commonly used as a phenotypic marker for murine T cells. |
UniProt Number | P22646 |
Product Page Updated | 2024-01-03T12:18:20.318Z |