CD15
Specifications
| Country Availability | all |
| BioSite Brand | BioSite Flow |
| Clone | MEM-158 |
| Clone Type | monoclonal |
| Description | Mouse Monoclonal to CD15 / Lewis x Blood Group antigen |
| Recommended Dilution | The purified antibody is conjugated with Peridinin-chlorophyll-protein complex (PerCP) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary. |
| Supplier | BioSite Flow |
| Immunogen | Human granulocytes |
| Isotype | IgM |
| Keywords | Blood Group Antigens (Human), CD and Related Antigens (Human) |
| Notes | CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells. There are several variants of Lewis x, such as sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression has been extensively used to confirm diagnosis of Hodgkin´s disease. |
| Species Reactivity | human |
| Storage | Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label. |
| Substrate / Buffer | The reagent is provided in stabilizing Tris buffered saline (TBS) solution containing 15 mM sodium azide. |
| Technical Specifications | The antibody MEM-158 reacts with CD15, a cell membrane molecule 3-fucosyl-N-acetyllactosamine (3-FAL) strongly expressed on granulocytes, monocytes, macrophages, mast cells; it is also present on Langerhans cells and some myeloid precursors cells.<br>_x000D_ <b>HLDA VI; WS Code AS A053</b> |
| Product Page Updated | 2018-07-27T09:12:01.609Z |
Shipping info
The delivery time for this item is approximately 5-8 business days. If other deviations occur, this will be noted on the order confirmation or communicated via email. Please note, we do reserve the right to select the best packaging and shipping method in order to insure the stability of the product and allow efficient order tracking.
Species reactivity:
human
Suggested protocols:
Immunoprecipitation