Article No
AFC-82YF8A-0.1
Specifications
| Application | FC, WB |
| Article No | AFC-82YF8A-0.1 |
| Biosite Brand | Biosite Flow |
| Country Availability | all |
| Clone | EM-53 |
| Clone Type | monoclonal |
| Concentration | 1 mg/ml |
| Conjugation | Unconjugated |
| Description | Anti-RLTPR Purified |
| Supplier | Nordic Biosite |
| Entrez Gene ID | 146206 |
| Format | Purified |
| Immunogen | Murine RLTPR |
| Isotype | IgG1 k |
| Keywords | Cytoskeleton: Actin System (Human), Cytoskeleton: Actin System (Rodent) |
| Notes | RLTPR / CARMIL2 (RGD motif, leucine rich repeats, tropomodulin domain and proline-rich containing; capping protein regulator and myosin 1 linker 2), also known as LRRC16C, is a cytosolic protein, which with high affinity binds CAPZA2 (capping protein muscle actin Z-line alpha 2) and decreases CAPZA2 affinity for actin barbed ends. RLTPR / CARMIL2 increases the rate of actin filament elongation from seeds in the presence of CAPZA2, however, seems unable to nucleate filaments. Its interaction with CAPZA2 is essential for lamellipodial protrusion and cell translocation. RLTPR / CARMIL2 is crutial for T cell costimulation via CD28 and this property seems to be independent on its actin-uncapping function. The lack of functional RLTPR / CARMIL2 molecules impeded the differentiation toward Th1 and Th17 fates of both human and murine CD4+ T cells and leads to combined immunodeficiency. Expression of RLTPR / CARMIL2 was also detected in human and murine B cells, but it seems not to be involved in BCR-mediated signaling. |
| Alias Names | CARMIL2, LRRC16C |
| Previous Article No | AFC-53CA53, AFC-82YF8A-0.1 |
| Product Type | Antibodies Primary |
| Protocol | Flow cytometry: Recommended dilution: 1-4 µg/ml. Intracellular staining. |
| Purification | Purified by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Size | 0.1 mg |
| Source / Host | mouse |
| Species Reactivity | mouse, human |
| Storage | Store at 2-8°C. Do not freeze. |
| Substrate / Buffer | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Technical Specifications | The mouse monoclonal antibody EM-53 recognizes RLTPR / CARMIL2, an intracellular protein playing a role in actin filament elongation. |
| UniProt Number | Q6F5E8 |
| Product Page Updated | 2024-01-03T12:18:20.318Z |
