RLTPR / CARMIL2

Article No AFC-53CA53
Product Type Antibodies Primary
Application FC, WB
Size 0.1 mg

Specifications

Country Availability all
BioSite Brand BioSite Flow
Clone EM-53
Clone Type monoclonal
Conjugation Unconjugated
Description Mouse Monoclonal to RLTPR / CARMIL2
Supplier BioSite Flow
Immunogen Murine Rltpr (amino acids 1147-1397)
Isotype IgG1
Keywords Cytoskeleton: Actin System (Human), Cytoskeleton: Actin System (Rodent)
Notes RLTPR / CARMIL2 (RGD motif, leucine rich repeats, tropomodulin domain and proline-rich containing; capping protein regulator and myosin 1 linker 2), also known as LRRC16C, is a cytosolic protein, which with high affinity binds CAPZA2 (capping protein muscle actin Z-line alpha 2) and decreases CAPZA2 affinity for actin barbed ends. RLTPR / CARMIL2 increases the rate of actin filament elongation from seeds in the presence of CAPZA2, however, seems unable to nucleate filaments. Its interaction with CAPZA2 is essential for lamellipodial protrusion and cell translocation. RLTPR / CARMIL2 is crutial for T cell costimulation via CD28 and this property seems to be independent on its actin-uncapping function. The lack of functional RLTPR / CARMIL2 molecules impeded the differentiation toward Th1 and Th17 fates of both human and murine CD4+ T cells and leads to combined immunodeficiency. Expression of RLTPR / CARMIL2 was also detected in human and murine B cells, but it seems not to be involved in BCR-mediated signaling.
Purification Purified from hybridoma culture supernatant by protein-A affinity chromatography.
Purity > 95% (by SDS-PAGE)
Species Reactivity human, mouse
Storage Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Substrate / Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Technical Specifications The mouse monoclonal antibody EM-53 recognizes RLTPR / CARMIL2, a protein playing a role in actin filament elongation.
Product Page Updated 2018-07-27T09:12:01.609Z

Shipping info

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Species reactivity:

human, mouse human mouse

Suggested protocols:

Western Blot

Western Blot