Article No
AFC-VK7MXU-100
Specifications
| Application | FC |
| Article No | AFC-VK7MXU-100 |
| Biosite Brand | Biosite Flow |
| Country Availability | all |
| Clone | dG9 |
| Clone Type | monoclonal |
| Conjugation | PE |
| Description | Anti-Perforin PE |
| Recommended Dilution | The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary. |
| Supplier | Nordic Biosite |
| Entrez Gene ID | 5551 |
| Format | PE |
| Immunogen | purified granules from human YT lymphoma cell line |
| Isotype | IgG2b k |
| Keywords | Apoptosis (Veterinary), Apoptosis (Human) |
| Notes | Perforin is a 70 kDa cytolytic protein with structural and functional similarities to complement component 9 (C9). It is stored in cytoplasmic granules of cytotoxic T cells and NK cells and after its release it forms transmembrane pores in the target cells to kill it. As perforin is a key effector molecule for cell-mediated cytolysis, defects of its gene can cause severe disorders. |
| Alias Names | PRF1, P1, PFP, HPLH2 |
| Previous Article No | AFC-5332-2 |
| Product Type | Antibodies Primary |
| Protocol | Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. Intracellular staining. |
| Purification | Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
| Research area | Cell Death |
| Size | 100 tests |
| Source / Host | mouse |
| Species Reactivity | human, cow/bovine |
| Storage | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
| Substrate / Buffer | Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Technical Specifications | The mouse monoclonal antibody dG9 (also known as deltaG9) recognizes perforin, a 70 kDa protein expressed in cytoplasmic granules of cytotoxic T cells and NK cells. |
| UniProt Number | P14222 |
| Product Page Updated | 2024-01-03T12:18:20.318Z |
