Article No
AFC-CH1R0E-100
Application | FC |
Article No | AFC-CH1R0E-100 |
Biosite Brand | Biosite Flow |
Country Availability | all |
Clone | HM47 |
Clone Type | monoclonal |
Conjugation | PE |
Description | Anti-Hu CD79a PE |
Recommended Dilution | The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary. |
Supplier | Nordic Biosite |
Entrez Gene ID | 973 |
Format | PE |
Immunogen | Synthetic peptide corresponding to C terminal amino acids 208-222 of human CD79a |
Isotype | IgG1 k |
Keywords | CD Antigens & MHC (Veterinary), CD and Related Antigens & MHC (Rodent), CD and Related Antigens (Human) |
Notes | CD79a (Ig alpha, MB1) forms disulfide-linked heterodimer with CD79b (Ig beta). They both are transmembrane proteins with extended cytoplasmic domains containing immunoreceptor tyrosine activation motives (ITAMs), and together with cell surface immunoglobulin they constitute B-cell antigen-specific receptor (BCR). CD79a and b are the first components of BCR that are expressed developmentally. They appear on pro-B cells in association with the endoplasmic reticulum chaperone calnexin. Subsequently, in pre-B cells, CD79 heterodimer is associated with lambda5-VpreB surrogate immunoglobulin and later with antigen-specific surface immunoglobulins. At the plasma cell stage, CD79a is present as an intracellular component. CD79a/b complex interacts with Src-family tyrosine kinase Lyn, which phosphorylates its cytoplasmic ITAM motives to form docking sites for downstream signaling. |
Alias Names | BCR alpha, Ig-alpha, MB-1, IGA |
Previous Article No | AFC-5315-2 |
Product Type | Antibodies Primary |
Protocol | Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. Intracellular staining. |
Purification | Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
Research Area | Immunology |
Size | 100 tests |
Source / Host | mouse |
Species Reactivity | dog/canine, chicken, rabbit, guinea pig, horse/equine, cow/bovine, mouse, rat, pig/porcine, monkey, human |
Storage | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
Substrate / Buffer | Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Technical Specifications | The mouse monoclonal antibody HM47 reacts with intracellular domain of CD79a (Ig alpha), a 40-45 kDa subunit of B cell antigen-specific receptor (BCR) and its early developmental forms. |
UniProt Number | P11912 |
Product Page Updated | 2024-01-03T12:18:20.318Z |