Article No
AFC-I78D00-100
Application | FC |
Article No | AFC-I78D00-100 |
Biosite Brand | Biosite Flow |
Country Availability | all |
Clone | H4A3 |
Clone Type | monoclonal |
Conjugation | PE |
Description | Anti-Hu CD107a PE |
Recommended Dilution | The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary. |
Supplier | Nordic Biosite |
Entrez Gene ID | 3916 |
Format | PE |
Immunogen | Human PBMC |
Isotype | IgG1 k |
Keywords | CD Antigens & MHC (Veterinary), CD and Related Antigens & MHC (Rodent), CD and Related Antigens (Human) |
Notes | CD107a (lysosome-associated membrane protein-1, LAMP-1), together with LAMP-2, is a major constituent of lysosomal membrane, 1-2% of total CD107a is found also on the plasma membrane. The LAMP proteins are involved in lysosome biogenesis and are required for fusion of lysosomes with phagosomes. Increased CD107a immunoreactivity is observed in neurones, and in glial cells surrounding senile plaques in Alzheimers disease cases and is localized mainly in medullary epithelial cells, single macrophages and lymphocytes in acute thymic involution. CD107a is a good marker of mast cell activation. |
Alias Names | LAMP-1, LAMPA |
Previous Article No | AFC-5279-2 |
Product Type | Antibodies Primary |
Protocol | Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. Intracellular and extracellular staining. |
Purification | Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
Research area | Immunology |
Size | 100 tests |
Source / Host | mouse |
Species Reactivity | human, monkey, mouse |
Storage | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
Substrate / Buffer | Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Technical Specifications | The mouse monoclonal antibody H4A3 recognizes an extracellular/luminal epitope of CD107a, an approximately 100-120 kDa glycoprotein expressed mainly on lysosomal, but also on the plasma membrane. |
UniProt Number | P11279 |
Product Page Updated | 2024-01-03T12:18:20.318Z |