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Anti-Hu CD163 PE

Antibodies Primary

Make a customized order

Article No

AFC-J5WMO0-100

Application

Species Reactivity

human

Size

100 tests

Clone

GHI/61

Source / Host

mouse

Article No

AFC-J5WMO0-100

Application

Species Reactivity

human

Size

100 tests

Clone

GHI/61

Source / Host

mouse

Specifications
Suggested protocols
References

Specifications

Application	FC
Article No	AFC-J5WMO0-100
Biosite Brand	Biosite Flow
Country Availability	all
Clone	GHI/61
Clone Type	monoclonal
Conjugation	PE
Description	Anti-Hu CD163 PE
Recommended Dilution	The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
Supplier	Nordic Biosite
Entrez Gene ID	9332
Format	PE
Immunogen	Hairy cell leukemia cells
Isotype	IgG1 k
Keywords	CD and Related Antigens (Human)
Notes	CD163, also known as M130, is a member of the scavenger receptor family, accounting for the clearance of hemoglobin-haptoglobin complexes during limited hemolysis, which protects the body, in particular the kidneys, against heme-mediated oxidative damages. It does not have measurable affinity for noncomplexed hemoglobin or haptoglobin. Immunomodulatory role of CD163 has been postulated. CD163 is expressed by cells of the monocyte-macrophage lineage and its extracellular part also circulates in plasma as a soluble protein, especially during sepsis and other conditions affecting macrophage activity, when its level may raise manyfold.
Alias Names	MM130, SCARI1
Previous Article No	AFC-5255-2
Product Type	Antibodies Primary
Protocol	Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Purification	Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Research area	Immunology
Size	100 tests
Source / Host	mouse
Species Reactivity	human
Storage	Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Substrate / Buffer	Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Technical Specifications	The mouse monoclonal antibody GHI/61 recognizes an extracellular epitope CD163, an approximately 130 kDa high affinity scavenger receptor expressed mainly on monocytes and macrophages, which binds hemoglobin-haptoglobin complex.
UniProt Number	Q86VB7
Product Page Updated	2024-01-03T12:18:20.318Z

Suggested protocols

Flow Cytometry

References

*Ohradanova-Repic A, Machacek C, Charvet C, Lager F, Le Roux D, Platzer R, Leksa V, Mitulovic G, Burkard TR, Zlabinger GJ, Fischer MB, Feuillet V, Renault G, Blüml S, Benko M, Suchanek M, Huppa JB, Matsuyama T, Cavaco-Paulo A, Bismuth G, Stockinger H: Extracellular Purine Metabolism Is the Switchboard of Immunosuppressive Macrophages and a Novel Target to Treat Diseases With Macrophage Imbalances. Front Immunol. 2018 Apr 27;9:852., URL: https://www.ncbi.nlm.nih.gov/pubmed/29780382 ,*Pulford K, Micklem K, McCarthy S, Cordell J, Jones M, Mason DY: A monocyte/macrophage antigen recognized by the four antibodies GHI/61, Ber-MAC3, Ki-M8 and SM4. Immunology. 1992 Apr;75(4):588-95., URL: http://www.ncbi.nlm.nih.gov/pubmed/1592433 ,*Kusi KA, Gyan BA, Goka BQ, Dodoo D, Obeng-Adjei G, Troye-Blomberg M, Akanmori BD, Adjimani JP: Levels of soluble CD163 and severity of malaria in children in Ghana. Clin Vaccine Immunol. 2008 Sep;15(9):1456-60., URL: http://www.ncbi.nlm.nih.gov/pubmed/18632918 ,*Bover LC, Cardó-Vila M, Kuniyasu A, Sun J, Rangel R, Takeya M, Aggarwal BB, Arap W, Pasqualini R: A previously unrecognized protein-protein interaction between TWEAK and CD163: potential biological implications. J Immunol. 2007 Jun 15;178(12):8183-94., URL: http://www.ncbi.nlm.nih.gov/pubmed/17548657 ,*Moniuszko M, Kowal K, Rusák M, Pietruczuk M, Dabrowska M, Bodzenta-Lukaszyk A: Monocyte CD163 and CD36 expression in human whole blood and isolated mononuclear cell samples: influence of different anticoagulants. Clin Vaccine Immunol. 2006 Jun;13(6):704-7., URL: https://www.ncbi.nlm.nih.gov/pubmed/16760331,*Kim WK, Alvarez X, Fisher J, Bronfin B, Westmoreland S, McLaurin J, Williams K: CD163 identifies perivascular macrophages in normal and viral encephalitic brains and potential precursors to perivascular macrophages in blood. Am J Pathol. 2006 Mar;168(3):822-34., URL: https://www.ncbi.nlm.nih.gov/pubmed/16507898,*Philippidis P, Mason JC, Evans BJ, Nadra I, Taylor KM, Haskard DO, Landis RC: Hemoglobin scavenger receptor CD163 mediates interleukin-10 release and heme oxygenase-1 synthesis: antiinflammatory monocyte-macrophage responses in vitro, in resolving skin blisters in vivo, and after cardiopulmonary bypass surgery. Circ Res. 2004 Jan 9;94(1):119-26., URL: https://www.ncbi.nlm.nih.gov/pubmed/14656926,*Madsen M, Møller HJ, Nielsen MJ, Jacobsen C, Graversen JH, van den Berg T, Moestrup SK: Molecular characterization of the haptoglobin.hemoglobin receptor CD163. Ligand binding properties of the scavenger receptor cysteine-rich domain region. J Biol Chem. 2004 Dec 3;279(49):51561-7., URL: https://www.ncbi.nlm.nih.gov/pubmed/15448162,*Møller HJ, Peterslund NA, Graversen JH, Moestrup SK: Identification of the hemoglobin scavenger receptor/CD163 as a natural soluble protein in plasma. Blood. 2002 Jan 1;99(1):378-80., URL: https://www.ncbi.nlm.nih.gov/pubmed/11756196,*Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997)., URL: ,*Law SK, Micklem KJ, Shaw JM, Zhang XP, Dong Y, Willis AC, Mason DY: A new macrophage differentiation antigen which is a member of the scavenger receptor superfamily. Eur J Immunol. 1993 Sep;23(9):2320-5., URL: https://www.ncbi.nlm.nih.gov/pubmed/8370408,*Pulford K, Micklem K, McCarthy S, Cordell J, Jones M, Mason DY: A monocyte/macrophage antigen recognized by the four antibodies GHI/61, Ber-MAC3, Ki-M8 and SM4. Immunology. 1992 Apr;75(4):588-95., URL: https://www.ncbi.nlm.nih.gov/pubmed/1592433,,,

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