Article No
AFC-SWFCPS-100
Specifications
| Application | FC |
| Article No | AFC-SWFCPS-100 |
| Biosite Brand | Biosite Flow |
| Country Availability | all |
| Clone | 16A1 |
| Clone Type | monoclonal |
| Conjugation | PE |
| Description | Anti-Hu CD140a PE |
| Recommended Dilution | The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary. |
| Supplier | Nordic Biosite |
| Entrez Gene ID | 5156 |
| Format | PE |
| Immunogen | CD140a-transfected NIH 3T3 cells |
| Isotype | IgG1 k |
| Keywords | Cytokine Signaling (Human), CD and Related Antigens (Human) |
| Notes | CD140a / PDGF-RA (platelet-derived growth factor receptor alpha) is a cell surface receptor for members of platelet-derived growth factor family, whose intracellular part contains a tyrosine kinase domain. CD140a forms homodimers, or heterodimerizes with CD140b / PDGF-RB. Whereas CD140b induces in different cell types their proliferation and migration, the role of CD140a is more controversial, with pro-proliferative or anti-proliferative effects. CD140a has early developmental functions, mediates mesodermal cell migration, and later acts in signaling associated in epithelial-mesenchymal interactions. |
| Alias Names | PDGFRA, PDGFR2 |
| Previous Article No | AFC-5233-2 |
| Product Type | Antibodies Primary |
| Protocol | Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests. |
| Purification | Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
| Research area | Cell Signaling |
| Size | 100 tests |
| Source / Host | mouse |
| Species Reactivity | human |
| Storage | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
| Substrate / Buffer | Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Technical Specifications | The mouse monoclonal antibody 16A1 recognizes an extracellular epitope of CD140a / PDGF-RA, the 170 kDa alpha chain of platelet-derived growth factor receptor, which is widely expressed on a variety of mesenchymal-derived cells and plays pro-proliferative or anti-proliferative roles in various tumours. |
| UniProt Number | P16234 |
| Product Page Updated | 2024-01-03T12:18:20.318Z |
