Article No
AFC-Z3G3WV-100
Application | FC |
Article No | AFC-Z3G3WV-100 |
Biosite Brand | Biosite Flow |
Country Availability | all |
Clone | 104D2 |
Clone Type | monoclonal |
Conjugation | PE |
Description | Anti-Hu CD117 PE |
Recommended Dilution | The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary. |
Supplier | Nordic Biosite |
Entrez Gene ID | 3815 |
Format | PE |
Immunogen | MOLM-1 megakaryocytic cells |
Isotype | IgG1 k |
Keywords | Phosphorylation (Kinases, Phosphatases, Phosphotyrosine) (Veterinary), CD Antigens & MHC (Veterinary), Phosphorylation (Kinases, Phosphatases, Phosphotyrosine) (Human), CD and Related Antigens (Human) |
Notes | CD117 / c-Kit (stem cell factor receptor) is a 145 kDa receptor tyrosine kinase that regulates cell proliferation, adhesion, chemotaxis, apoptosis and other cell processes. Mutations of CD117 / c-Kit can lead to growth and progression of tumours. After binding of its ligand, SCF (stem cell factor), CD117 / c-Kit is autophosphorylated on its intracellular domains and activated. CD117 is expressed on pluripotent hematopoietic progenitor cells, mast cells and various cancer cells, e.g. acute myeloid leukemia cells. |
Alias Names | c-Kit, PBT, SCFR |
Previous Article No | AFC-5230-2 |
Product Type | Antibodies Primary |
Protocol | Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests. |
Purification | Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
Size | 100 tests |
Source / Host | mouse |
Species Reactivity | monkey, human, cow/bovine |
Storage | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
Substrate / Buffer | Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Technical Specifications | The mouse monoclonal antibody 104D2 detects extracellular part of CD117 / c-Kit protooncogen. |
UniProt Number | P10721 |
Product Page Updated | 2024-01-03T12:18:20.318Z |