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Anti-Hu CD28 PE

Antibodies Primary

Make a customized order

Article No

AFC-QFP12R-100

Application

Size

100 tests

Clone

CD28.2

Source / Host

mouse

Species Reactivity

monkey

human

Article No

AFC-QFP12R-100

Application

Size

100 tests

Clone

CD28.2

Source / Host

mouse

Species Reactivity

monkey

human

Specifications
Suggested protocols
References

Specifications

Application	FC
Article No	AFC-QFP12R-100
Biosite Brand	Biosite Flow
Country Availability	all
Clone	CD28.2
Clone Type	monoclonal
Conjugation	PE
Description	Anti-Hu CD28 PE
Recommended Dilution	The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
Supplier	Nordic Biosite
Entrez Gene ID	940
Format	PE
Immunogen	DC28.1.3.3 murine T cell hybridoma transfected with human CD28 cDNA
Isotype	IgG1 k
Keywords	CD Antigens & MHC (Veterinary), CD and Related Antigens (Human)
Notes	CD28 is the critical T cell costimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its costimulation role CD28 functions in preventing T cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. CD28 is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader.
Alias Names	TP44
Previous Article No	AFC-5223-2
Product Type	Antibodies Primary
Protocol	Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
Purification	Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Research area	Immunology
Size	100 tests
Source / Host	mouse
Species Reactivity	monkey, human
Storage	Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Substrate / Buffer	Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Technical Specifications	The mouse monoclonal antibody CD28.2 recognizes an extracellular epitope of CD28, a disulfide-linked homodimeric type I glycoprotein (monomer of Mw 44 kDa) which is a critical costimulatory receptor of T cells.
UniProt Number	P10747
Product Page Updated	2024-01-03T12:18:20.318Z

Suggested protocols

Flow Cytometry

References

*Marti F, Krause A, Post NH, Lyddane C, Dupont B, Sadelain M, King PD: Negative-feedback regulation of CD28 costimulation by a novel mitogen-activated protein kinase phosphatase, MKP6. J Immunol. 2001 Jan 1;166(1):197-206. , URL: https://www.ncbi.nlm.nih.gov/pubmed/11123293?ordin,*Scharschmidt E, Wegener E, Heissmeyer V, Rao A, Krappmann D: Degradation of Bcl10 induced by T-cell activation negatively regulates NF-kappa B signaling. Mol Cell Biol. 2004 May;24(9):3860-73. , URL: https://www.ncbi.nlm.nih.gov/pubmed/15082780?ordin,*Jeong SH, Qiao M, Nascimbeni M, Hu Z, Rehermann B, Murthy K, Liang TJ. Immunization with hepatitis C virus-like particles induces humoral and cellular immune responses in nonhuman primates. J Virol. 2004 Jul;78(13):6995-7003., URL: https://www.ncbi.nlm.nih.gov/pubmed/15194776?ordin,*Nunès J, Klasen S, Ragueneau M, Pavon C, Couez D, Mawas C, Bagnasco M, Olive D: CD28 mAbs with distinct binding properties differ in their ability to induce T cell activation: analysis of early and late activation events. Int Immunol. 1993 Mar;5(3):311-5., URL: https://www.ncbi.nlm.nih.gov/pubmed/8385476?ordina,*Nunes J, Klasen S, Franco MD, Lipcey C, Mawas C, Bagnasco M, Olive D: Signalling through CD28 T-cell activation pathway involves an inositol phospholipid-specific phospholipase C activity. Biochem J. 1993 Aug 1;293 ( Pt 3):835-42., URL: https://www.ncbi.nlm.nih.gov/pubmed/8394695?ordina,*Schlossman, S., et al., Eds. 1995. Leucocyte Typing V. Oxford University Press. New York., URL: ,*Galea-Lauri J, Darling D, Gan SU, Krivochtchapov L, Kuiper M, Gäken J, Souberbielle B, Farzaneh F: Expression of a variant of CD28 on a subpopulation of human NK cells: implications for B7-mediated stimulation of NK cells. J Immunol. 1999 Jul 1;163(1):62-70., URL: https://www.ncbi.nlm.nih.gov/pubmed/10384100?ordin,*Tazi A, Moreau J, Bergeron A, Dominique S, Hance AJ, Soler P: Evidence that Langerhans cells in adult pulmonary Langerhans cell histiocytosis are mature dendritic cells: importance of the cytokine microenvironment. J Immunol. 1999 Sep 15;163(6):3511-5., URL: https://www.ncbi.nlm.nih.gov/pubmed/10477625?ordin,*Mary C, Coulon F, Poirier N, Dilek N, Martinet B, Blancho G, Vanhove B: Antagonist properties of monoclonal antibodies targeting human CD28: Role of valency and the heavy-chain constant domain. MAbs Jan-Feb 2013;5(1):47-55., URL: https://pubmed.ncbi.nlm.nih.gov/23221503/,,,

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