CD45 (mouse)

Article No AFC-5217-2
Product Type Antibodies Primary
Application FC, IP, WB, ICC
Size 0.1 mg

Specifications

Country Availability all
BioSite Brand BioSite Flow
Clone EM-05
Clone Type monoclonal
Concentration 0.5 mg/ml
Conjugation PE
Description Rat Monoclonal to CD45 (mouse)
Recommended Dilution The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Supplier BioSite Flow
Immunogen Murine peripheral blood leukocytes_x000D_
Isotype IgG
Keywords Phosphorylation (Kinases, Phosphatases, Phosphotyrosine) (Rodent), CD and Related Antigens & MHC (Rodent)
Notes CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis.
Species Reactivity mouse
Storage Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Substrate / Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Technical Specifications The antibody EM-05 reacts with mouse CD45 antigen (Leukocyte Common Antigen), a single chain type I transmembrane protein expressed at high level on cells of hematopoietic origin, except erythrocytes and platelets.
Product Page Updated 2018-07-27T09:12:01.609Z

Shipping info

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Species reactivity:

mouse mouse