CD14

Article No AFC-5148-2
Product Type Antibodies Primary
Research Area Immunology
Application FC, IP
Size 100 tests

Specifications

Country Availability all
BioSite Brand BioSite Flow
Clone MEM-15
Clone Type monoclonal
Conjugation PE
Description Mouse Monoclonal to CD14 / LPS-receptor
Recommended Dilution The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
Supplier BioSite Flow
Immunogen A crude mixture of human urinary proteins precipitated by ammonium sulphate from the urine of a patient suffering from proteinuria.
Isotype IgG1
Keywords CD Antigens & MHC (Veterinary), CD and Related Antigens (Human)
Notes CD14 is a 55 kDa GPI-anchored glycoprotein, constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils, where serves as a multifunctional lipopolysaccharide receptor; it is also released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein. The soluble sCD14 is able to discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles. CD14 affects allergic, inflammatory and infectious processes.
Species Reactivity human, monkey
Storage Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Substrate / Buffer The reagent is provided in stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide.
Technical Specifications The antibody MEM-15 reacts with CD14, a 53-55 kDa GPI (glycosylphosphatidylinositol)-linked membrane glycoprotein expressed on monocytes, macrophages and weakly on granulocytes; also expressed by most tissue macrophages. <br>The antibody MEM-15 also reacts with soluble forms of CD14 found in serum and in the urine of some nephrotic patients. <br>_x000D_ <b>HLDA III; WS Code M 252<br>_x000D_ HLDA IV; WS Code M 113<br>_x000D_ HLDA IV; WS Code NL 90<br>_x000D_ HLDA IV; WS Code T 53<br>_x000D_ HLDA V; WS Code M MA086<br>_x000D_ HLDA VI; WS Code M MA94</b>
Product Page Updated 2018-07-27T09:12:01.609Z

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Species reactivity:

human, monkey human monkey

Suggested protocols:

Immunoprecipitation

Immunoprecipitation