Article No
AFC-7IJ9ML-0.1
Application | FC |
Article No | AFC-7IJ9ML-0.1 |
Biosite Brand | Biosite Flow |
Country Availability | all |
Clone | B2M-01 |
Clone Type | monoclonal |
Concentration | 0.1 mg/ml |
Conjugation | PE |
Description | Anti-Hu beta2-Microglobulin PE |
Recommended Dilution | The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography. |
Supplier | Nordic Biosite |
Entrez Gene ID | 567 |
Format | PE |
Immunogen | Purified human beta2-microglobulin |
Isotype | IgG2a |
Keywords | Serum & Plasma Proteins (Human), MHC (Major Histocompatibility Complex) and Related Antigens (Human) |
Notes | Beta2-microglobulin non-covalently associates with the 44 kDa alpha chain to forms the HLA Class I antigen complex. Human beta2-microglobulin associated with HLA Class I antigens is expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes. |
Alias Names | B2M |
Previous Article No | AFC-5137-2, AFC-7IJ9ML-0.1 |
Product Type | Antibodies Primary |
Protocol | Flow cytometry: Recommended dilution: 1-2 μg/ml; positive control: peripheral blood lymphocytes, negative control: DAUDI human Burkitt lymphoma cell line, erythrocytes. |
Purification | Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
Size | 0.1 mg |
Source / Host | mouse |
Species Reactivity | human |
Storage | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
Substrate / Buffer | Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Technical Specifications | The mouse monoclonal antibody B2M-01 reacts with beta2-microglobulin (beta2M; extracellular antigen), associated with cell-surface MHC Class I molecules and other membrane antigens, as well as it reacts with soluble beta2-microglobulin. Beta2M is a 12 kDa Ig like glycoprotein expressed on lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells and epithelial cells. It is absent on erythrocytes. |
UniProt Number | P61769 |
Product Page Updated | 2024-01-03T12:18:20.318Z |