Article No
AFC-2R0WVR-0.1
Mouse IgG2a Isotype Control FITC
Mouse IgG2a Isotype Control FITC
Antibodies Isotype Control
Specifications
| Application | FC |
| Article No | AFC-2R0WVR-0.1 |
| Biosite Brand | Biosite Flow |
| Country Availability | all |
| Clone | MOPC-173 |
| Clone Type | monoclonal |
| Concentration | 1 mg/ml |
| Conjugation | FITC |
| Description | Mouse IgG2a Isotype Control FITC |
| Recommended Dilution | The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC. |
| Supplier | Nordic Biosite |
| Format | FITC |
| Immunogen | The transplantable plasmacytoma MOPC-173 was induced by intraperitoneal injection of mineral oils into BALB/c mice. |
| Isotype | IgG2a k |
| Keywords | Immunoglobulin Isotype Controls |
| Notes | The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 μg/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody MOPC-173, generated against an undefined antigen, does not react specifically with mouse, rat and human samples, and hence all the background that could be observed when working with this antibody would be a result of general nonspecific interactions between an mouse IgG2a molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished. |
| Previous Article No | AFC-5087-2, AFC-2R0WVR-0.1 |
| Product Type | Antibodies Isotype Control |
| Protocol | Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 μg/ml of incubation mixture), change the conditions of your experiment to reduce the background. |
| Purification | Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
| Size | 0.1 mg |
| Source / Host | mouse |
| Storage | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
| Substrate / Buffer | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Technical Specifications | This mouse IgG2a monoclonal antibody (clone MOPC-173) reacts with an unknown epitope. It does not react with a variety of resting, activated, live, and fixed mouse, rat and human tissues. |
| Product Page Updated | 2024-01-03T12:18:20.318Z |
