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Anti-Rt CD8b FITC

Antibodies Primary

Make a customized order

Article No

AFC-BF39KM-0.1

Application

Species Reactivity

rat

Size

0.1 mg

Clone

341

Source / Host

mouse

Article No

AFC-BF39KM-0.1

Application

Species Reactivity

rat

Size

0.1 mg

Clone

341

Source / Host

mouse

Specifications
Suggested protocols
References

Specifications

Application	FC
Article No	AFC-BF39KM-0.1
Biosite Brand	Biosite Flow
Country Availability	all
Clone	341
Clone Type	monoclonal
Concentration	0.5 mg/ml
Conjugation	FITC
Description	Anti-Rt CD8b FITC
Recommended Dilution	The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC.
Supplier	Nordic Biosite
Entrez Gene ID	926
Format	FITC
Immunogen	CD8 positive Wistar rat splenic T cell hybridomas
Isotype	IgG1 k
Keywords	CD and Related Antigens & MHC (Rodent)
Notes	The CD8b (CD8 beta) subunit of CD8 T cell coreceptor is expressed in CD8 alpha/beta heterodimers on majority of MHC I-restricted conventional T cells and thymocytes and in CD8 alpha/alpha homodimers on subsets of memory T cells, intraepithelial lymphocytes, NK cells, macrophages, mast cells, and dendritic cells. Regulation of CD8 beta level on T cell surface seems to be an important mechanism to control their effector function. Assembly of CD8 alpha/beta but not alpha/alpha dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling.
Alias Names	Ly-3, Ly-C, Lyt-3
Previous Article No	AFC-5058-2, AFC-BF39KM-0.1
Product Type	Antibodies Primary
Protocol	Flow cytometry: Recommended dilution: 1-4 μg/ml.
Purification	Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Research area	Immunology
Size	0.1 mg
Source / Host	mouse
Species Reactivity	rat
Storage	Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Substrate / Buffer	Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Technical Specifications	The mouse monoclonal antibody 341 (also known as 34.1) recognizes rat CD8b, the 32-34 kDa beta chain of the CD8 coreceptor (extracellular epitope), expressed on T cell subsets and some other cell types, such as macrophages.
UniProt Number	P10966
Product Page Updated	2024-01-03T12:18:20.318Z

Suggested protocols

Flow Cytometry

References

*Torres-Nagel N, Kraus E, Brown MH, Tiefenthaler G, Mitnacht R, Williams AF, Hünig T: Differential thymus dependence of rat CD8 isoform expression. Eur J Immunol. 1992 Nov;22(11):2841-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/1358623,*Kraus E, Lambracht D, Wonigeit K, Hünig T: Negative regulation of rat natural killer cell activity by major histocompatibility complex class I recognition. Eur J Immunol. 1996 Nov;26(11):2582-6., URL: https://www.ncbi.nlm.nih.gov/pubmed/8921942,*Nohara O, Kulka M, Déry RE, Wills FL, Hirji NS, Gilchrist M, Befus AD: Regulation of CD8 expression in mast cells by exogenous or endogenous nitric oxide. J Immunol. 2001 Nov 15;167(10):5935-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/11698471,*Ellerman KE, Like AA: Islet cell membrane antigens activate diabetogenic CD4+ T-cells in the BB/Wor rat. Diabetes. 1999 May;48(5):975-82., URL: https://www.ncbi.nlm.nih.gov/pubmed/10331400,*Lin TJ, Hirji N, Nohara O, Stenton GR, Gilchrist M, Befus AD: Mast cells express novel CD8 molecules that selectively modulate mediator secretion. J Immunol. 1998 Dec 1;161(11):6265-72., URL: https://www.ncbi.nlm.nih.gov/pubmed/9834115,*Hirji N, Lin TJ, Bissonnette E, Belosevic M, Befus AD: Mechanisms of macrophage stimulation through CD8: macrophage CD8alpha and CD8beta induce nitric oxide production and associated killing of the parasite Leishmania major. J Immunol. 1998 Jun 15;160(12):6004-11., URL: https://www.ncbi.nlm.nih.gov/pubmed/9637515,*Hirji N, Lin TJ, Befus AD: A novel CD8 molecule expressed by alveolar and peritoneal macrophages stimulates nitric oxide production. J Immunol. 1997 Feb 15;158(4):1833-40., URL: https://www.ncbi.nlm.nih.gov/pubmed/9029123,*Kühnlein P, Park JH, Herrmann T, Elbe A, Hünig T: Identification and characterization of rat gamma/delta T lymphocytes in peripheral lymphoid organs, small intestine, and skin with a monoclonal antibody to a constant determinant of the gamma/delta T cell receptor. J Immunol. 1994 Aug 1;153(3):979-86., URL: https://www.ncbi.nlm.nih.gov/pubmed/8027567,*Mabarrack NH, Turner NL, Mayrhofer G: Recent thymic origin, differentiation, and turnover of regulatory T cells. J Leukoc Biol. 2008 Nov;84(5):1287-97., URL: https://www.ncbi.nlm.nih.gov/pubmed/18682578,,,

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