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Anti-Ms CD154 FITC

Antibodies Primary

Make a customized order

Article No

AFC-F2X3S3-0.1

Application

Species Reactivity

mouse

Size

0.1 mg

Clone

MR-1

Source / Host

mouse

Article No

AFC-F2X3S3-0.1

Application

Species Reactivity

mouse

Size

0.1 mg

Clone

MR-1

Source / Host

mouse

Specifications
Suggested protocols
References

Specifications

Application	FC
Article No	AFC-F2X3S3-0.1
Biosite Brand	Biosite Flow
Country Availability	all
Clone	MR-1
Clone Type	monoclonal
Concentration	0.5 mg/ml
Conjugation	FITC
Description	Anti-Ms CD154 FITC
Recommended Dilution	The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC.
Supplier	Nordic Biosite
Entrez Gene ID	959
Format	FITC
Immunogen	Activated mouse Th1 clone D1.6
Isotype	IgG1
Keywords	CD and Related Antigens & MHC (Rodent)
Notes	CD154 / CD40L (CD40 ligand) is a member of the tumor necrosis factor family, and is expressed primarily on activated CD4+ lymphocytes, but also on mast cells, basophils, eosinophils and human dendritic cells. Its counter-receptor CD40 is expressed on antigen presenting cells, including dendritic cells, macrophages, and B cells, and also on fibroblasts. Triggering of CD40 by CD40L causes maturation of dendritic cells and upregulation of antigen presentation in functions of the MHC and costimulatory molecules. CD40L also functions as a direct stimulating factor for T cells. CD40L plays also roles e.g. in antibody class switching and modulation of apoptosis in the germinal center.
Alias Names	CD40L, CD40 ligand, TNFSF5, Ly62, gp39, T-BAM
Previous Article No	AFC-5046-2, AFC-F2X3S3-0.1
Product Type	Antibodies Primary
Protocol	Flow cytometry: Recommended dilution: 2-3 μg/ml; positive control: 6-8 hour activated murine splenocytes.
Purification	Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Research area	Immunology
Size	0.1 mg
Source / Host	mouse
Species Reactivity	mouse
Storage	Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Substrate / Buffer	Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Technical Specifications	The Armenian hamster monoclonal antibody MR-1 detects an extracellular epitope on murine CD154 / CD40L (CD40-ligand), a 39 kDa cell surface type II glycoprotein expressed predominantly on activated CD4+ lymphocytes.
UniProt Number	P29965
Product Page Updated	2024-01-03T12:18:20.318Z

Suggested protocols

Flow Cytometry

References

*Lode HN, Xiang R, Pertl U, Förster E, Schoenberger SP, Gillies SD, Reisfeld RA: Melanoma immunotherapy by targeted IL-2 depends on CD4(+) T-cell help mediated by CD40/CD40L interaction. J Clin Invest. 2000 Jun;105(11):1623-30., URL: https://www.ncbi.nlm.nih.gov/pubmed/10841521?itool,*Fischbein MP, Ardehali A, Yun J, Schoenberger S, Laks H, Irie Y, Dempsey P, Cheng G, Fishbein MC, Bonavida B: CD40 signaling replaces CD4+ lymphocytes and its blocking prevents chronic rejection of heart transplants. J Immunol. 2000 Dec 15;165(12):7316-22., URL: https://www.ncbi.nlm.nih.gov/pubmed/11120867?itool,*Hwang Y, Nahm MH, Briles DE, Thomas D, Purkerson JM: Acquired, but not innate, immune responses to Streptococcus pneumoniae are compromised by neutralization of CD40L. Infect Immun. 2000 Feb;68(2):511-7., URL: https://www.ncbi.nlm.nih.gov/pubmed/10639411?itool,*Murakawa T, Kerklo MM, Zamora MR, Wei Y, Gill RG, Henson PM, Grover FL, Nicolls MR: Simultaneous LFA-1 and CD40 ligand antagonism prevents airway remodeling in orthotopic airway transplantation: implications for the role of respiratory epithelium as a modulator of fibrosis. J Immunol. 2005 Apr 1;174(7):3869-79., URL: https://www.ncbi.nlm.nih.gov/pubmed/15778341?itool,*Salgado CG, Nakamura K, Sugaya M, Tada Y, Asahina A, Koyama Y, Irie S, Tamaki K: Functional CD40 ligand is expressed on epidermal Langerhans cells. J Leukoc Biol. 1999 Aug;66(2):281-5., URL: https://www.ncbi.nlm.nih.gov/pubmed/10449168?itool,*Wu Y, Zhou Q, Zheng P, Liu Y: CD28-independent induction of T helper cells and immunoglobulin class switches requires costimulation by the heat-stable antigen. J Exp Med. 1998 Apr 6;187(7):1151-6., URL: https://www.ncbi.nlm.nih.gov/pubmed/9529332?itool=,*Luzina IG, Atamas SP, Storrer CE, daSilva LC, Kelsoe G, Papadimitriou JC, Handwerger BS: Spontaneous formation of germinal centers in autoimmune mice. J Leukoc Biol. 2001 Oct;70(4):578-84., URL: https://www.ncbi.nlm.nih.gov/pubmed/11590194?itool,*Shin T, Kennedy G, Gorski K, Tsuchiya H, Koseki H, Azuma M, Yagita H, Chen L, Powell J, Pardoll D, Housseau F: Cooperative B7-1/2 (CD80/CD86) and B7-DC costimulation of CD4+ T cells independent of the PD-1 receptor. J Exp Med. 2003 Jul 7;198(1):31-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/12847135?itool,*Howard LM, Miga AJ, Vanderlugt CL, Dal Canto MC, Laman JD, Noelle RJ, Miller SD: Mechanisms of immunotherapeutic intervention by anti-CD40L (CD154) antibody in an animal model of multiple sclerosis. J Clin Invest. 1999 Jan;103(2):281-90., URL: https://www.ncbi.nlm.nih.gov/pubmed/9916140?itool=,*Noelle RJ, Roy M, Shepherd DM, Stamenkovic I, Ledbetter JA, Aruffo A: A 39-kDa protein on activated helper T cells binds CD40 and transduces the signal for cognate activation of B cells. Proc Natl Acad Sci U S A. 1992 Jul 15;89(14):6550-4., URL: https://www.ncbi.nlm.nih.gov/pubmed/1378631?itool=,,,

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