Article No
AFC-F2CYQ6-0.1
Specifications
| Application | FC |
| Article No | AFC-F2CYQ6-0.1 |
| Biosite Brand | Biosite Flow |
| Country Availability | all |
| Clone | DR5-01-1 |
| Clone Type | monoclonal |
| Concentration | 0.1 mg/ml |
| Conjugation | FITC |
| Description | Anti-Hu CD262 FITC |
| Recommended Dilution | The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC. |
| Supplier | Nordic Biosite |
| Entrez Gene ID | 8795 |
| Format | FITC |
| Immunogen | Recombinant fusion protein of human IgG heavy chain and extracellular domain of DR5. |
| Isotype | IgG1 |
| Keywords | Apoptosis (Human), CD and Related Antigens (Human), Apoptosis |
| Notes | TRAIL-R2 (CD262, DR5) is one of two TNF superfamily member intracellular death domain containing receptors for TRAIL (APO2L). Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including tumor necrosis factor (TNF) and Fas ligand in the TNF family through their death domain containing receptors, TNF receptor 1 (TNFR1) and Fas, respectively. Another member in the TNF family has been identified and designated TRAIL (for TNF related apoptosis inducing ligand) and Apo2L (for Apo2 ligand). Receptors for TRAIL include two death domain containing receptors, DR4 and DR5, as well as two decoy receptors, DcR1 and DcR2, lacking the intracellular signaling death domain. DcR1 (also called TRID), like the related death receptors DR4 and DR5, contains two extracellular cysteine rich domains. However, DcR1 contains no intracellular death domain and is thus incapable of signaling apoptosis. It has been suggested DcR1 is responsible for TRAIL resistance in normal human tissues including heart, placenta, lung, liver, kidney, spleen, and bone marrow. DR5 is a member of the TNF receptor superfamily, and contains an intracellular death domain. This receptor can be activated by tumor necrosis factor related apoptosis inducing ligand (TNFSF10/TRAIL/APO2L), and transduces apoptosis signal. Studies with FADD deficient mice suggested that FADD, a death domain containing adaptor protein, is required for the apoptosis mediated by this protein. |
| Alias Names | TNFRSF10B, KILLER, TRICK2, TRAIL-R2 |
| Previous Article No | AFC-5006-2, AFC-F2CYQ6-0.1 |
| Product Type | Antibodies Primary |
| Protocol | Flow cytometry: Recommended dilution: 1-5 μg/ml; positive control: JURKAT human peripheral blood leukemia T cell line. |
| Purification | Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
| Research area | Cell Death |
| Size | 0.1 mg |
| Source / Host | mouse |
| Species Reactivity | human |
| Storage | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
| Substrate / Buffer | Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Technical Specifications | The mouse monoclonal antibody DR5-01-1 recognizes an extracellular domain of TRAIL-R2 (DR5). TRAIL-R2 is one of two TNF superfamily members that contain death domain for TRAIL (APO2L). |
| UniProt Number | O14763 |
| Product Page Updated | 2024-01-03T12:18:20.318Z |
