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Anti-Hu CD107a PerCP-Cy™5.5

Antibodies Primary

Make a customized order

Article No

AFC-4FV4Q4-100

Application

Size

100 tests

Clone

H4A3

Source / Host

mouse

Species Reactivity

human

monkey

mouse

Article No

AFC-4FV4Q4-100

Application

Size

100 tests

Clone

H4A3

Source / Host

mouse

Species Reactivity

human

monkey

mouse

Specifications
Suggested protocols
References

Specifications

Application	FC
Article No	AFC-4FV4Q4-100
Biosite Brand	Biosite Flow
Country Availability	all
Clone	H4A3
Clone Type	monoclonal
Conjugation	PerCP/Cy5.5
Description	Anti-Hu CD107a PerCP-Cy™5.5
Supplier	Nordic Biosite
Entrez Gene ID	3916
Format	PerCP-Cy5.5
Immunogen	Human PBMC
Isotype	IgG1 k
Notes	CD107a (lysosome-associated membrane protein-1, LAMP-1), together with LAMP-2, is a major constituent of lysosomal membrane, 1-2% of total CD107a is found also on the plasma membrane. The LAMP proteins are involved in lysosome biogenesis and are required for fusion of lysosomes with phagosomes. Increased CD107a immunoreactivity is observed in neurones, and in glial cells surrounding senile plaques in Alzheimers disease cases and is localized mainly in medullary epithelial cells, single macrophages and lymphocytes in acute thymic involution. CD107a is a good marker of mast cell activation.
Alias Names	LAMP-1, LAMPA
Product Type	Antibodies Primary
Protocol	Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. Intracellular and extracellular staining.
Purification	Purified antibody is conjugated with activated tandem dye of activated Peridinin-Chlorophyll Protein-Cyanine 5.5 (PerCP-Cy™5.5) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Size	100 tests
Source / Host	mouse
Species Reactivity	human, monkey, mouse
Storage	Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Substrate / Buffer	Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Technical Specifications	The mouse monoclonal antibody H4A3 recognizes an extracellular/luminal epitope of CD107a, an approximately 100-120 kDa glycoprotein expressed mainly on lysosomal, but also on the plasma membrane.
UniProt Number	P11279
Product Page Updated	2024-01-03T12:18:20.318Z

Suggested protocols

Flow Cytometry

References

*Majer F, Vlaskova H, Krol L, Kalina T, Kubanek M, Stolnaya L, Dvorakova L, Elleder M, Sikora J: Danon disease: a focus on processing of the novel LAMP2 mutation and comments on the beneficial use of peripheral white blood cells in the diagnosis of LAMP2 deficiency. Gene. 2012 May 1;498(2):183-95., URL: https://pubmed.ncbi.nlm.nih.gov/22365987/,*Mao H, Tu W, Liu Y, Qin G, Zheng J, Chan PL, Lam KT, Peiris JS, Lau YL: Inhibition of human natural killer cell activity by influenza virions and hemagglutinin. J Virol. 2010 May;84(9):4148-57., URL: http://www.ncbi.nlm.nih.gov/pubmed/20164232 ,*Yu CI, Gallegos M, Marches F, Zurawski G, Ramilo O, García-Sastre A, Banchereau J, Palucka AK: Broad influenza-specific CD8+ T-cell responses in humanized mice vaccinated with influenza virus vaccines. Blood. 2008 Nov 1;112(9):3671-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/18713944,*Tomescu C, Chehimi J, Maino VC, Montaner LJ: NK cell lysis of HIV-1-infected autologous CD4 primary T cells: requirement for IFN-mediated NK activation by plasmacytoid dendritic cells. J Immunol. 2007 Aug 15;179(4):2097-104., URL: https://www.ncbi.nlm.nih.gov/pubmed/17675468,*Carlsten M, Björkström NK, Norell H, Bryceson Y, van Hall T, Baumann BC, Hanson M, Schedvins K, Kiessling R, Ljunggren HG, Malmberg KJ: DNAX accessory molecule-1 mediated recognition of freshly isolated ovarian carcinoma by resting natural killer cells. Cancer Res. 2007 Feb 1;67(3):1317-25., URL: https://www.ncbi.nlm.nih.gov/pubmed/17283169,*Marcenaro S, Gallo F, Martini S, Santoro A, Griffiths GM, Aricó M, Moretta L, Pende D: Analysis of natural killer-cell function in familial hemophagocytic lymphohistiocytosis (FHL): defective CD107a surface expression heralds Munc13-4 defect and discriminates between genetic subtypes of the disease. Blood. 2006 Oct 1;108(7):2316-23., URL: https://www.ncbi.nlm.nih.gov/pubmed/16778144,*Deetz CO, Hebbeler AM, Propp NA, Cairo C, Tikhonov I, Pauza CD: Gamma interferon secretion by human Vgamma2Vdelta2 T cells after stimulation with antibody against the T-cell receptor plus the Toll-Like receptor 2 agonist Pam3Cys. Infect Immun. 2006 Aug;74(8):4505-11., URL: https://www.ncbi.nlm.nih.gov/pubmed/16861636,*Furuta K, Ikeda M, Nakayama Y, Nakamura K, Tanaka M, Hamasaki N, Himeno M, Hamilton SR, August JT: Expression of lysosome-associated membrane proteins in human colorectal neoplasms and inflammatory diseases. Am J Pathol. 2001 Aug;159(2):449-55., URL: https://www.ncbi.nlm.nih.gov/pubmed/11485903,*Mane SM, Marzella L, Bainton DF, Holt VK, Cha Y, Hildreth JE, August JT: Purification and characterization of human lysosomal membrane glycoproteins. Arch Biochem Biophys. 1989 Jan;268(1):360-78., URL: https://pubmed.ncbi.nlm.nih.gov/2912382/,,*Grützkau A, Smorodchenko A, Lippert U, Kirchhof L, Artuc M, Henz BM: LAMP-1 and LAMP-2, but not LAMP-3, are reliable markers for activation-induced secretion of human mast cells. Cytometry A. 2004 Sep;61(1):62-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/15351990?ordin,*Barrachina M, Maes T, Buesa C, Ferrer I: Lysosome-associated membrane protein 1 (LAMP-1) in Alzheimer's disease. Neuropathol Appl Neurobiol. 2006 Oct;32(5):505-16., URL: https://www.ncbi.nlm.nih.gov/pubmed/16972884?ordin,*Sarafian VS, Marinova TT: Lysosomal membrane-associated glycoproteins are differentially expressed in acute and chronic human thymic involution. Acta Biol Hung. 2006 Sep;57(3):315-22., URL: https://www.ncbi.nlm.nih.gov/pubmed/17048695?ordin,*Eskelinen EL: Roles of LAMP-1 and LAMP-2 in lysosome biogenesis and autophagy. Mol Aspects Med. 2006 Oct-Dec;27(5-6):495-502., URL: https://www.ncbi.nlm.nih.gov/pubmed/16973206?ordin,*Hunyh KK, Eskelinen EL, Scott CC, Malevanets A, Saftig P, Grinstein S: LAMP proteins are required for fusion of lysosomes with phagosomes. EMBO J. 2007 Jan 24;26(2):313-24., URL: https://www.ncbi.nlm.nih.gov/pubmed/17245426?ordin,,

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