Article No
AFC-67NINC-100
Anti-Hu CD235ab FITC
Anti-Hu CD235ab FITC
Antibodies Primary
Specifications
| Application | FC |
| Article No | AFC-67NINC-100 |
| Biosite Brand | Biosite Flow |
| Country Availability | all |
| Clone | HIR2 |
| Clone Type | monoclonal |
| Conjugation | FITC |
| Description | Anti-Hu CD235ab FITC |
| Recommended Dilution | The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use. No reconstitution is necessary. |
| Supplier | Nordic Biosite |
| Entrez Gene ID | 2993 |
| Format | FITC |
| Immunogen | Synthetic peptide (Human, N-terminal) |
| Isotype | IgG2b |
| Keywords | CD and Related Antigens (Human) |
| Notes | CD235a (glycophorin A, GPA) is a transmembrane sialoglycoprotein expressed on erythrocytes and their precursors. Similarly to CD235b (glycophorin B, GPB), these molecules provide the cells with a large mucin-like surface, which minimalizes aggregation between erythrocytes in the circulation. GPA is the carrier of blood group M and N specificities, while GPB accounts for S, s and U specificities. CD235a is a receptor of Hsa, a Streptococcus adhesin. |
| Alias Names | Glycophorin A/B, GYPA/B, GPA/B, PAS-2/3, Sialoglycoprotein alpha/beta, GPSAT, GPErik, MSN blood group |
| Previous Article No | AFC-4995-2 |
| Product Type | Antibodies Primary |
| Protocol | Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests. |
| Purification | Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
| Research area | Immunology |
| Size | 100 tests |
| Source / Host | mouse |
| Species Reactivity | human |
| Storage | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
| Substrate / Buffer | Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Technical Specifications | The mouse monoclonal antibody HIR2 recognizes the N-terminal (extracellular) portion of glycophorin A (and weakly of glycophorin B). Its antigen is expressed on early erythroblasts, late erythroblasts, erythroblasts, mature erythrocytes and the cells of erythroid cell lines K562 and HEL, but not on all other cells. |
| UniProt Number | P02724 |
| Product Page Updated | 2024-01-03T12:18:20.318Z |
