Article No
AFC-YWLZP3-50
Specifications
| Application | FC |
| Article No | AFC-YWLZP3-50 |
| Biosite Brand | Biosite Flow |
| Country Availability | all |
| Clone | Hs-14 |
| Clone Type | monoclonal |
| Conjugation | FITC |
| Description | Anti-VCP FITC |
| Recommended Dilution | The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use. No reconstitution is necessary. |
| Supplier | Nordic Biosite |
| Entrez Gene ID | 7415 |
| Format | FITC |
| Immunogen | Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization. |
| Isotype | IgM |
| Keywords | Fertility & Reproduction (Rodent), Fertility & Reproduction (Human), Fertility & Reproduction |
| Notes | VCP (valosin-containing protein), also known as p97, TERA, ALS14, IBMPFD, HEL-220, IBMPFD1, or HEL-S-70, is a member of a protein family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. VCP is a structural protein that associates with clathrin and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation. In sperm this intra-acrosomal protein can be used as a marker for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction. |
| Alias Names | TERA, CDC48 |
| Previous Article No | AFC-4969-1 |
| Product Type | Antibodies Primary |
| Protocol | Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 50 tests. Intraacrosomal staining. |
| Purification | Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
| Size | 50 tests |
| Source / Host | mouse |
| Species Reactivity | mouse, human |
| Storage | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
| Substrate / Buffer | Stabilizing Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide |
| Technical Specifications | The antibody Hs-14 reacts with VCP (valosin-containing protein) a 220 kDa intra-acrosomal protein. |
| UniProt Number | P55072 |
| Product Page Updated | 2024-01-03T12:18:20.318Z |
