CD15

Article No AFC-4935-2
Product Type Antibodies Primary
Research Area Immunology
Application FC, IP
Size 100 tests

Specifications

Country Availability all
BioSite Brand BioSite Flow
Clone MEM-158
Clone Type monoclonal
Conjugation FITC
Description Mouse Monoclonal to CD15 / Lewis x Blood Group antigen
Recommended Dilution The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use. No reconstitution is necessary.
Supplier BioSite Flow
Immunogen Human granulocytes
Isotype IgM
Keywords Blood Group Antigens (Human), CD and Related Antigens (Human)
Notes CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells. There are several variants of Lewis x, such as sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression has been extensively used to confirm diagnosis of Hodgkin´s disease.
Species Reactivity human
Storage Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Substrate / Buffer The reagent is provided in stabilizing Tris buffered saline (TBS) solution containing 15 mM sodium azide.
Technical Specifications The antibody MEM-158 reacts with CD15, a cell membrane molecule 3-fucosyl-N-acetyllactosamine (3-FAL) strongly expressed on granulocytes, monocytes, macrophages, mast cells; it is also present on Langerhans cells and some myeloid precursors cells.<br>_x000D_ <b>HLDA VI; WS Code AS A053</b>
Product Page Updated 2018-07-27T09:12:01.609Z

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Species reactivity:

human human

Suggested protocols:

Immunoprecipitation

Immunoprecipitation