IgG2b Isotype Control

Article No AFC-4913-2
Product Type Antibodies Isotype Control
Application FC, IP, WB, IHC-P, IHC-Fr, ICC
Size 0.1 mg

Specifications

Country Availability all
BioSite Brand BioSite Flow
Clone MPC-11
Clone Type monoclonal
Concentration 1 mg/ml
Conjugation Biotin
Description Mouse IgG2b Isotype Control
Recommended Dilution The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.
Supplier BioSite Flow
Immunogen KLH-coupled trinitrophenol
Isotype IgG2b
Keywords Immunoglobulin Isotype Controls
Notes The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 μg/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody MPC-11 was generated against an epitope irrelevant for human, mouse, and rat material, and can thus be used for evaluation of the background staining that is caused by general nonspecific interactions between an mouse IgG2b molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished.
Storage Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Substrate / Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Technical Specifications This mouse IgG2b (kappa) monoclonal antibody (clone MPC-11) reacts with an epitope irrelevant for a variety of resting, activated, live, and fixed human, mouse, and rat tissues.
Product Page Updated 2018-07-27T09:12:01.609Z

Shipping info

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