Article No
AFC-KTY6HY-0.1
Application | FC, IP, IHC, ICC |
Article No | AFC-KTY6HY-0.1 |
Biosite Brand | Biosite Flow |
Country Availability | all |
Clone | ICRF44 |
Clone Type | monoclonal |
Concentration | 1 mg/ml |
Conjugation | Biotin |
Description | Anti-Hu CD11b Biotin |
Recommended Dilution | The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin. |
Supplier | Nordic Biosite |
Entrez Gene ID | 3684 |
Format | Biotin |
Immunogen | Rheumatoid synovial cells and human monocytes. |
Isotype | IgG1 |
Keywords | CD and Related Antigens (Human) |
Notes | CD11b (integrin alphaM subunit) is a 165-170 kDa type I transmembrane glycoprotein that non-covalently associates with integrin beta2 subunit (CD18); expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages. CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component. |
Alias Names | Mac-1, Integrin alpha M, ITGAM, CR3A, MO1A, MAC1A |
Previous Article No | AFC-4911-2, AFC-KTY6HY-0.1 |
Product Type | Antibodies Primary |
Protocol | Flow cytometry: Recommended dilution: 1-12 μg/ml. |
Purification | Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography. |
Research Area | Immunology |
Size | 0.1 mg |
Source / Host | mouse |
Species Reactivity | human, monkey |
Storage | Store at 2-8°C. Do not freeze. |
Substrate / Buffer | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Technical Specifications | The mouse monoclonal antibody ICRF44 recognizes an extracellular epitope of CD11b (Mac-1alpha), a 165-170 kDa type 1 transmembrane protein mainly expressed on monocytes, granulocytes and NK-cells. |
UniProt Number | P11215 |
Product Page Updated | 2024-01-03T12:18:20.318Z |