Article No
AFC-A2TIYS-0.1
Application | FC, IP, WB, IHC, ICC |
Article No | AFC-A2TIYS-0.1 |
Biosite Brand | Biosite Flow |
Country Availability | all |
Clone | 10.1 |
Clone Type | monoclonal |
Concentration | 1 mg/ml |
Conjugation | Biotin |
Description | Anti-Hu CD64 Biotin |
Recommended Dilution | The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin. |
Supplier | Nordic Biosite |
Entrez Gene ID | 2209 |
Format | Biotin |
Immunogen | Rheumatoid synovial fluid cells and fibronectin purified human monocytes |
Isotype | IgG1 k |
Keywords | CD and Related Antigens (Human) |
Notes | CD64 (FcgammaRI) is a cell surface receptor for Fc region of IgG. It is composed of specific ligand binding alpha subunit and promiscuous gamma subunit, which is indispensable for tyrosine-based signaling. However, even the alpha subunit can transduce signals leading to cellular effector functions. The isoform FcgammaRIa1 binds human IgG with high affinity, has limited myeloid cell distribution, and a relatively large intracellular domain. Products of related genes include FcgammaRIb and FcgammaRIc isoforms, but these specify low affinity IgG receptors if functionally expressed at all. Besides a role in antigen clearance, FcgammaRI (a1) can potently enhance MHC class I and II antigen presentation in vitro and in vivo. |
Alias Names | FcRI, IGFR1, FcgR1A |
Previous Article No | AFC-4906-2, AFC-A2TIYS-0.1 |
Product Type | Antibodies Primary |
Protocol | Flow cytometry: Recommended dilution: 4 μg/ml. |
Purification | Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography. |
Research Area | Immunology |
Size | 0.1 mg |
Source / Host | mouse |
Species Reactivity | human, monkey |
Storage | Store at 2-8°C. Do not freeze. |
Substrate / Buffer | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Technical Specifications | The mouse monoclonal antibody 10.1 recognizes an extracellular epitope on CD64/FcgammaRI, a 72 kDa single chain type I glycoprotein, that is expressed on monocytes/macrophages, dendritic cells, and activated granulocytes. |
UniProt Number | P12314 |
Product Page Updated | 2024-01-03T12:18:20.318Z |