Article No
AFC-8SHJ8U-0.1
Application | FC, IP, IHC |
Article No | AFC-8SHJ8U-0.1 |
Biosite Brand | Biosite Flow |
Country Availability | all |
Clone | 2H7 |
Clone Type | monoclonal |
Concentration | 1 mg/ml |
Conjugation | Biotin |
Description | Anti-Hu CD20 Biotin |
Recommended Dilution | The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin. |
Supplier | Nordic Biosite |
Entrez Gene ID | 931 |
Format | Biotin |
Immunogen | Human tonsillar B cells |
Isotype | IgG2b k |
Keywords | CD Antigens & MHC (Veterinary), Calcium Signaling (Human), CD and Related Antigens (Human) |
Notes | CD20 is a cell surface 33-37 (depending on the degree of phosphorylation) kDa non-glycosylated surface phosphoprotein expressed on mature and most malignant B cells, but not stem cells or plasma cells (low number of the CD20 has been also detected on a subpopulation of T lymphocytes and it can be expressed on follicular dendritic cells). Its expression on B cells is synchronous with the expression of surface IgM. CD20 regulates transmembrane calcium conductance (probably functioning as a component of store-operated calcium channel), cell cycle progression and B-cell proliferation. It is associated with lipid rafts, but the intensity of this association depends on extracellular triggering, employing CD20 conformational change and/or BCR (B cell antigen receptor) aggregation. After the receptor ligation, BCR and CD20 colocalize and then rapidly dissociate before BCR endocytosis, whereas CD20 remains at the cell surface. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B cells, as it is expressed at high levels on most B-cell malignancies, but does not become internalized or shed from the plasma membrane following mAb treatment. |
Alias Names | B1, S7, MS4A, Bp35, CVID5, LEU-16 |
Previous Article No | AFC-4904-2, AFC-8SHJ8U-0.1 |
Product Type | Antibodies Primary |
Protocol | Flow cytometry: Recommended dilution: 1 μg/ml. |
Purification | Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography. |
Research area | Immunology |
Size | 0.1 mg |
Source / Host | mouse |
Species Reactivity | human, monkey |
Storage | Store at 2-8°C. Do not freeze. |
Substrate / Buffer | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Technical Specifications | The mouse monoclonal antibody 2H7 recognizes an extracellular epitope on CD20 (B1, Bp35), a 33-37 kDa non-glycosylated membrane receptor with four transmembrane domains, expressed on pre-B lymphocytes, resting and activated B cells (not plasma cells), follicular dendritic cells, and at low levels on peripheral blood T lymphocytes. |
UniProt Number | P11836 |
Product Page Updated | 2024-01-03T12:18:20.318Z |