Article No
AFC-QI2OGT-0.1
Application | FC, IP, IHC, ICC, ELISA |
Article No | AFC-QI2OGT-0.1 |
Biosite Brand | Biosite Flow |
Country Availability | all |
Clone | W6/32 |
Clone Type | monoclonal |
Concentration | 1 mg/ml |
Conjugation | Biotin |
Description | Anti-HLA Class I Biotin |
Recommended Dilution | The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin. |
Supplier | Nordic Biosite |
Format | Biotin |
Immunogen | Membrane of human tonsil cells |
Isotype | IgG2a |
Keywords | CD Antigens & MHC (Veterinary), MHC (Major Histocompatibility Complex) and Related Antigens (Human) |
Notes | HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with an invariant beta2 microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumour defence. Human HLA-class I antigens are represented by HLA-A, HLA-B and HLA-C molecules. |
Previous Article No | AFC-4885-2, AFC-QI2OGT-0.1 |
Product Type | Antibodies Primary |
Protocol | Flow cytometry: Recommended dilution: 1-12 µg/ml |
Purification | Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography. |
Research Area | Immunology |
Size | 0.1 mg |
Source / Host | mouse |
Species Reactivity | cow/bovine, monkey, human, cat/feline |
Storage | Store at 2-8°C. Do not freeze. |
Substrate / Buffer | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Technical Specifications | The antibody W6/32 recognises an extracellular epitope of MHC Class I molecules (MHC Class Ia) that are expressed on the surface of all human nucleated cell types. The antibody W6/32 is a valuable reagent for analysing variations in HLA class I expression in different disease states e.g. liver disease, muscular dystrophy, inflammatory myopathy and other neuromuscular disorders. This antibody W6/32 is also suitable as a positive control for HLA tissue typing and crossmatching. |
Product Page Updated | 2024-01-03T12:18:20.318Z |