Article No
AFC-TD85B8-0.1
Application | FC, IP |
Article No | AFC-TD85B8-0.1 |
Biosite Brand | Biosite Flow |
Country Availability | all |
Clone | MEM-171 |
Clone Type | monoclonal |
Concentration | 1 mg/ml |
Conjugation | Biotin |
Description | Anti-Hu CD50 Biotin |
Recommended Dilution | The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin. |
Supplier | Nordic Biosite |
Entrez Gene ID | 3385 |
Format | Biotin |
Immunogen | Human granulocytes |
Isotype | IgG1 |
Keywords | CD and Related Antigens (Human) |
Notes | CD50 (intracellular adhesion molecule 3, ICAM-3) is a transmembrane glycoprotein expressed by leukocytes, that serves as a counter-receptor for the lymphocyte function-associated antigen (LFA)-1 integrin. Besides functioning as an adhesive molecule that mediates e.g. the contact between T cells and antigen presenting cells, ICAM-3 regulates affinity of LFA-1 for ICAM-1 and induces T cell activation and proliferation. ICAM-3 plays an essential role in the initiation of the immune response both on T cells and antigen presenting cells and interacts also with CD209 (dendritic cell-specific ICAM-3-grabbing nonintegrin, DC-SIGN), a C-type lectin of dendritic cells and macrophages; this process is involved in dialogue between dendritic cells and granulocytes. |
Alias Names | ICAM-3, ICAM-R |
Previous Article No | AFC-4864-2, AFC-TD85B8-0.1 |
Product Type | Antibodies Primary |
Protocol | Flow cytometry: Recommended dilution: 3-5 μg/ml. |
Purification | Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography. |
Research Area | Immunology |
Size | 0.1 mg |
Source / Host | mouse |
Species Reactivity | human |
Storage | Store at 2-8°C. Do not freeze. |
Substrate / Buffer | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Technical Specifications | The antibody MEM-171 recognizes an extracellular epitope in the D2 domain of CD50 (ICAM-3), a 120-130 kDa type I membrane protein (immunoglobulin supergene family) expressed on leukocytes, endothelial cells and Langerhans cells; it is negative on platelets and erythrocytes. |
UniProt Number | P32942 |
Product Page Updated | 2024-01-03T12:18:20.318Z |