Article No
AFC-NVPPFI-0.1
Application | FC, IP, WB, ELISA |
Article No | AFC-NVPPFI-0.1 |
Biosite Brand | Biosite Flow |
Country Availability | all |
Clone | MEM-85 |
Clone Type | monoclonal |
Concentration | 1 mg/ml |
Conjugation | Biotin |
Description | Anti-Hu CD44 Biotin |
Recommended Dilution | The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin. |
Supplier | Nordic Biosite |
Entrez Gene ID | 960 |
Format | Biotin |
Immunogen | Leukocytes of a patient suffering from LGL Type Leukaemia. |
Isotype | IgG2b |
Keywords | CD and Related Antigens (Human) |
Notes | CD44 is a transmembrane glycoprotein expressed on the surface of most cells, which serves as a receptor for hyaluronan. CD44 mediates angiogenesis, cell adhesion, proliferation and migration, it is thus important for lymphocyte activation, recirculation and homing, it can thus serve e.g. as a modulator of macrophage recruitment in response to pathogen. Although CD44 functions are essential for physiological activities of normal cells, elevated CD44 expression correlates with poor prognosis in many carcinomas, facilitating tumour growth and metastasis, antiapoptosis and directional motility of cancer cells. |
Alias Names | PGP-I, HUTCH-I, ECMR-III, Hermes antigen, Hyaluronate receptor, Heparan sulfate proteoglycan, Epican, MC56, MIC4, INLU, LHR |
Previous Article No | AFC-4850-2, AFC-NVPPFI-0.1 |
Product Type | Antibodies Primary |
Protocol | Flow cytometry: Recommended dilution: 1-3 μg/ml. |
Purification | Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography. |
Research Area | Immunology |
Size | 0.1 mg |
Source / Host | mouse |
Species Reactivity | human |
Storage | Store at 2-8°C. Do not freeze. |
Substrate / Buffer | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Technical Specifications | The antibody MEM-85 reacts with an extracellular antigen of both cell surface-expressed and soluble form of CD44 antigen (Phagocyte glycoprotein 1), a 80-95 kDa transmembrane glycoprotein (hyaladherin family) present on the most of cells and tissues (leukocytes, endothelial cells, mesenchymal cells, etc.); it is negative on platelets and hepatocytes. |
UniProt Number | P16070 |
Product Page Updated | 2024-01-03T12:18:20.318Z |