Article No
AFC-OL8B20-100
Specifications
| Application | FC |
| Article No | AFC-OL8B20-100 |
| Biosite Brand | Biosite Flow |
| Country Availability | all |
| Clone | 6H6 |
| Clone Type | monoclonal |
| Conjugation | APC |
| Description | Anti-Hu CD123 APC |
| Recommended Dilution | The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary. |
| Supplier | Nordic Biosite |
| Entrez Gene ID | 3563 |
| Format | APC |
| Immunogen | IL3 receptor alpha chain expressed on the surface of transiently transfected COS cells |
| Isotype | IgG1 |
| Keywords | CD and Related Antigens (Human), Cytokine Signaling (Human) |
| Notes | CD123 is the alpha chain of interleukin 3 receptor (IL-3R alpha). This subunit heterodimerizes with the interleukin 3 receptor beta chain (CD131), which is shared with other receptors. CD123 interacts with IL-3 specifically, but with low affinity, and association with the beta subunit confers high affinity binding to the receptor heterodimer. Both chains are required for signaling, but receptor activation and signal transduction depend on IL-3 binding to CD123 as the initial step. |
| Alias Names | IL3RA, MGC34174, hIL-3Ra |
| Previous Article No | AFC-4820-2 |
| Product Type | Antibodies Primary |
| Protocol | Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. |
| Purification | Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
| Find out more about our research areas Biochemical, Immunology, Molecular Biology and Pathology | Immunology |
| Size | 100 tests |
| Source / Host | mouse |
| Species Reactivity | human |
| Storage | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
| Substrate / Buffer | Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Technical Specifications | The mouse monoclonal antibody 6H6 recognizes an extracellular epitope of CD123 (interleukin 3 receptor alpha), a 60-70 kDa transmembrane protein expressed by myeloid precursors, megakaryocytes, macrophages, dendritic cells, mast cells, basophils, and some B cells. This antibody does not inhibit IL-3 binding to its receptor. |
| UniProt Number | P26951 |
| Product Page Updated | 2024-01-03T12:18:20.318Z |
