Article No
AFC-6XPNF0-100
Application | FC |
Article No | AFC-6XPNF0-100 |
Biosite Brand | Biosite Flow |
Country Availability | all |
Clone | NGFR5 |
Clone Type | monoclonal |
Conjugation | APC |
Description | Anti-Hu CD271 APC |
Recommended Dilution | The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary. |
Supplier | Nordic Biosite |
Entrez Gene ID | 4804 |
Format | APC |
Immunogen | Purified CD271 protein isolated from human melanoma cell line A875 |
Isotype | IgG1 |
Keywords | Neurobiology (Veterinary), Neurobiology (Human), CD Antigens & MHC (Veterinary), CD and Related Antigens (Human) |
Notes | CD271 / NGFR, also known as p75NGFR or p75NTR, is a 75 kDa low affinity receptor for the NGF (nerve growth factor), BDNF (brain-derived growth factor), and other neurotrophins, such as NT3 and NT4/5. Unlike other members of the tumor necrosis factor receptor superfamily of transmembrane proteins, CD271 has unique intracellular domain structure (lacks catalytic activity) and downstream signaling partners. Triggered by its ligands CD271 affects growth, differentiation, migration and death of the nervous system cells. |
Alias Names | TNFRSF16, NGFR, p75NTR, p75NGFR |
Previous Article No | AFC-4789-2 |
Product Type | Antibodies Primary |
Protocol | Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. Extracellular and intracellular staining. |
Purification | Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
Research Area | Neuroscience |
Size | 100 tests |
Source / Host | mouse |
Species Reactivity | human, rabbit, cat/feline, monkey, mammals |
Storage | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
Substrate / Buffer | Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Technical Specifications | The mouse monoclonal antibody NGFR5 (originally C34C) recognizes an intracellular epitope of CD271/NGFR, a 75 kDa transmembrane glycoprotein of the TNFR superfamily. The epitope is localized within ammino acids 1 - 160. |
UniProt Number | P08138 |
Product Page Updated | 2024-01-03T12:18:20.318Z |