IgG1 Isotype Control

Article No AFC-4785-2
Product Type Antibodies Isotype Control
Application FC, IP, WB, IHC-P, IHC-Fr
Size 0.1 mg

Specifications

Country Availability all
BioSite Brand BioSite Flow
Clone MOPC-21
Clone Type monoclonal
Concentration 0.1 mg/ml
Conjugation APC
Description Mouse IgG1 Isotype Control
Recommended Dilution The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Supplier BioSite Flow
Isotype IgG1
Keywords Immunoglobulin Isotype Controls
Notes The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 μg/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody MOPC-21, generated against an undefined antigen, does not react specifically with rat and human samples, and hence all the background that could be observed when working with this antibody would be a result of general nonspecific interactions between an mouse IgG1 molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished.
Storage Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Substrate / Buffer The reagent is provided in stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide.
Technical Specifications This mouse IgG1 kappa monoclonal antibody (clone MOPC-21) has unknown specificity and was chosen as an isotype control after screening on variety of resting, activated, live and fixed rat and human tissues.
Product Page Updated 2018-07-27T09:12:01.609Z

Shipping info

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