Article No
AFC-ZWHD8B-100
Application | FC |
Article No | AFC-ZWHD8B-100 |
Biosite Brand | Biosite Flow |
Country Availability | all |
Clone | 16A1 |
Clone Type | monoclonal |
Conjugation | APC |
Description | Anti-Hu CD140a APC |
Recommended Dilution | The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary. |
Supplier | Nordic Biosite |
Entrez Gene ID | 5156 |
Format | APC |
Immunogen | CD140a-transfected NIH 3T3 cells |
Isotype | IgG1 k |
Keywords | Cytokine Signaling (Human), CD and Related Antigens (Human) |
Notes | CD140a / PDGF-RA (platelet-derived growth factor receptor alpha) is a cell surface receptor for members of platelet-derived growth factor family, whose intracellular part contains a tyrosine kinase domain. CD140a forms homodimers, or heterodimerizes with CD140b / PDGF-RB. Whereas CD140b induces in different cell types their proliferation and migration, the role of CD140a is more controversial, with pro-proliferative or anti-proliferative effects. CD140a has early developmental functions, mediates mesodermal cell migration, and later acts in signaling associated in epithelial-mesenchymal interactions. |
Alias Names | PDGFRA, PDGFR2 |
Previous Article No | AFC-4773-2 |
Product Type | Antibodies Primary |
Protocol | Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. |
Purification | Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
Research area | Cell Signaling |
Size | 100 tests |
Source / Host | mouse |
Species Reactivity | human |
Storage | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
Substrate / Buffer | Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Technical Specifications | The mouse monoclonal antibody 16A1 recognizes an extracellular epitope of CD140a / PDGF-RA, the 170 kDa alpha chain of platelet-derived growth factor receptor, which is widely expressed on a variety of mesenchymal-derived cells and plays pro-proliferative or anti-proliferative roles in various tumours. |
UniProt Number | P16234 |
Product Page Updated | 2024-01-03T12:18:20.318Z |