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Anti-Ms CD9 APC

Antibodies Primary

Make a customized order

Article No

AFC-ZKUJPI-0.1

Application

Species Reactivity

mouse

Size

0.1 mg

Clone

EM-04

Source / Host

mouse

Article No

AFC-ZKUJPI-0.1

Application

Species Reactivity

mouse

Size

0.1 mg

Clone

EM-04

Source / Host

mouse

Specifications
Suggested protocols
References

Specifications

Application	FC
Article No	AFC-ZKUJPI-0.1
Biosite Brand	Biosite Flow
Country Availability	all
Clone	EM-04
Clone Type	monoclonal
Concentration	0.5 mg/ml
Conjugation	APC
Description	Anti-Ms CD9 APC
Recommended Dilution	The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Supplier	Nordic Biosite
Entrez Gene ID	12527
Format	APC
Immunogen	Permeabilized murine bone marrow-derived mast cells (BMMC).
Isotype	IgG1
Keywords	CD and Related Antigens & MHC (Rodent)
Notes	CD9 belongs to proteins of tetraspanin family that orchestrate cholesterol-associated tetraspanin-enriched signaling microdomains within the plasma membrane, forming complexes with each other as well as with integrins, membrane-anchored growth factors and other proteins. CD9 is involved in cell motility, osteoclastogenesis, neurite outgrowth, myotube formation, and sperm-egg fusion, plays roles in cell attachment and proliferation and is necessary for association of heterologous MHC II molecules on the dendritic cell plasma membrane which is important for effective T cell stimulation. CD9 is also considered as metastasis suppressor in solid tumors.
Alias Names	Mic3, Mrp-1, Btcc-1, Drap-27, Tspan-29
Previous Article No	AFC-4764-2, AFC-ZKUJPI-0.1
Product Type	Antibodies Primary
Protocol	Flow cytometry: Recommended dilution: 2-3 μg/ml.
Purification	Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Research area	Immunology
Size	0.1 mg
Source / Host	mouse
Species Reactivity	mouse
Storage	Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Substrate / Buffer	Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Technical Specifications	The rat monoclonal antibody EM-04 recognizes an extracellular epitope of CD9 antigen, a 24 kDa transmembrane protein expressed on platelets, monocytes, pre-B lymphocytes, granulocytes and activated T lymphocytes.
UniProt Number	P40240
Product Page Updated	2024-01-03T12:18:20.318Z

Suggested protocols

Flow Cytometry

References

*Athman JJ, Wang Y, McDonald DJ, Boom WH, Harding CV, Wearsch PA: Bacterial membrane vesicles mediate the release of Mycobacterium tuberculosis lipoglycans and lipoproteins from infected macrophages. J Immunol. 2015 Aug 1;195(3):1044-53., URL: https://www.ncbi.nlm.nih.gov/pubmed/?term=athman+jj+wang+y+boom+wh,,*Kotha J, Zhang C, Longhurst CM, Lu Y, Jacobs J, Cheng Y, Jennings LK: Functional relevance of tetraspanin CD9 in vascular smooth muscle cell injury phenotypes: a novel target for the prevention of neointimal hyperplasia. Atherosclerosis. 2009 Apr;203(2):377-86., URL: http://www.ncbi.nlm.nih.gov/pubmed/18799160?ordina,*Liu WM, Cao YJ, Yang YJ, Li J, Hu Z, Duan EK: Tetraspanin CD9 regulates invasion during mouse embryo implantation. J Mol Endocrinol. 2006 Feb;36(1):121-30., URL: https://www.ncbi.nlm.nih.gov/pubmed/16461932?ordin,*Saito Y, Tachibana I, Takeda Y, Yamane H, He P, Suzuki M, Minami S, Kijima T, Yoshida M, Kumagai T, Osaki T, Kawase I. Absence of CD9 enhances adhesion-dependent morphologic differentiation, survival, and matrix metalloproteinase-2 production in small cell lung cancer cells. Cancer Res. 2006 Oct 1;66(19):9557-65., URL: https://pubmed.ncbi.nlm.nih.gov/17018612/,*Israels SJ, McMillan-Ward EM: Platelet tetraspanin complexes and their association with lipid rafts. Thromb Haemost. 2007 Nov;98(5):1081-7., URL: https://pubmed.ncbi.nlm.nih.gov/18000614/,*Kim YJ, Yu JM, Joo HJ, Kim HK, Cho HH, Bae YC, Jung JS: Role of CD9 in proliferation and proangiogenic action of human adipose-derived mesenchymal stem cells. Pflugers Arch. 2007 Nov;455(2):283-96., URL: https://pubmed.ncbi.nlm.nih.gov/17668233/,*Unternaehrer JJ, Chow A, Pypaert M, Inaba K, Mellman I: The tetraspanin CD9 mediates lateral association of MHC class II molecules on the dendritic cell surface. Proc Natl Acad Sci U S A. 2007 Jan 2;104(1):234-9., URL: https://pubmed.ncbi.nlm.nih.gov/17190803/,,

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