Article No
AFC-H4VLS5-0.1
Application | FC |
Article No | AFC-H4VLS5-0.1 |
Biosite Brand | Biosite Flow |
Country Availability | all |
Clone | MEM-G/9 |
Clone Type | monoclonal |
Concentration | 0.1 mg/ml |
Conjugation | APC |
Description | Anti-HLA-G APC |
Recommended Dilution | The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography. |
Supplier | Nordic Biosite |
Entrez Gene ID | 3135 |
Format | APC |
Immunogen | Recombinant human HLA-G refolded with beta2-microglobulin and peptide. |
Isotype | IgG1 |
Keywords | MHC (Major Histocompatibility Complex) and Related Antigens (Human) |
Notes | Human leukocyte antigen G (HLA-G), belonging to MHC class I glycoproteins, plays important roles in both physiological and pathological immunotolerance. It gives an inhibitory signal to cytotoxic T cells, NK cells, monocytes, and some other immune cells. It also induces regulatory T cells and anti-inflammatory macrophages. HLA-G is important e.g. for maternal tolerance to the fetus, and for immunomodulation in particular adult tissues, such as in cornea, pancreatic islets, thymus and other. On the other hand, it is expressed in many solid and hematologic malignancies, where it contributes to evasion of the immune surveillance. HLA-G expression pattern in cancer is an important prognostic factor regarding a poor clinical outcome. Unlike most other MHC glycoproteins, HLA-G acts as an immune checkpoint molecule rather than as an antigen presenting molecule. It concerns both transmembrane and soluble HLA-G isoforms. Among other, HLA-G can promote Th2 immunological response and downregulate Th1 immunological response. For its benefits regarding allograft tolerance, including embryo implantation, soluble HLA-G (sHLA-G) can be used as a marker of developmental potential of embryos during the process of in vitro fertilization. Similarly, sHLA-G concentrations in maternal serum are decreased in preeclampsia. Transplanted patients with increased sHLA-G serum levels have improved allograft acceptance. On the other hand, increased sHLA-G can also indicate presence of malignant (sometimes also of benign) tumor cells. Another important topic is induction of HLA-G expression (sometimes associated with shedding of HLA-G from the cell surface) by some anti-cancer or anti-viral therapies, which can weaken the therapy effect. Monitoring of HLA-G in patients thus has a wide usage. |
Previous Article No | AFC-4718-2, AFC-H4VLS5-0.1 |
Product Type | Antibodies Primary |
Protocol | Flow cytometry: Recommended dilution: 1-5 μg/ml; positive control: JEG-3 human choriocarcinoma cell line. |
Purification | Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
Research Area | Immunology |
Size | 0.1 mg |
Source / Host | mouse |
Species Reactivity | human |
Storage | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
Substrate / Buffer | Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Technical Specifications | The antibody MEM-G/9 reacts with an extracellular epitope on native form of human HLA-G1 on the cell surface as well as with soluble HLA-G5 isoform in its beta2-microglobulin associated form. Reactivity with HLA-G3 was also reported. The antibody MEM-G/9 is standard reagent thoroughly validated during 3rd International Conference on HLA-G (Paris, 2003). |
UniProt Number | P17693 |
Product Page Updated | 2024-01-03T12:18:20.318Z |