Article No
AFC-7LUONW-100
Application | FC |
Article No | AFC-7LUONW-100 |
Biosite Brand | Biosite Flow |
Country Availability | all |
Clone | MEM-55 |
Clone Type | monoclonal |
Conjugation | APC |
Description | Anti-Hu CD45RB APC |
Recommended Dilution | The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary. |
Supplier | Nordic Biosite |
Format | APC |
Immunogen | Human thymocytes and T lymphocytes. |
Isotype | IgG1 |
Keywords | Phosphorylation (Kinases, Phosphatases, Phosphotyrosine) (Human), CD Antigens & MHC (Veterinary), CD and Related Antigens (Human) |
Notes | CD45RB is an of a receptor-type protein tyrosine phosphatase, CD45 glycoprotein. CD45 is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45RB is expressed e.g. in microglia and inflammatory cells. |
Alias Names | PTPRCR, T200RB |
Previous Article No | AFC-4709-2 |
Product Type | Antibodies Primary |
Protocol | Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. |
Purification | Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
Size | 100 tests |
Source / Host | mouse |
Species Reactivity | monkey, human |
Storage | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
Substrate / Buffer | Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Technical Specifications | The antibody MEM-55 recognizes a siliadase-sensitive extracellular epitope of CD45RB, a 180-240 kDa single chain type I membrane glycoprotein, variant of CD45 (CD45RB isoform). CD45RB is expressed on a subset of T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells. |
Product Page Updated | 2024-01-03T12:18:20.318Z |