IgG2a Isotype Control

Article No AFC-4675-2
Product Type Antibodies Isotype Control
Application FC, IP, WB, IHC, ICC
Size 0.1 mg

Specifications

Country Availability all
BioSite Brand BioSite Flow
Clone MOPC-173
Clone Type monoclonal
Concentration 1 mg/ml
Description Mouse IgG2a Isotype Control
Supplier BioSite Flow
Immunogen The transplantable plasmacytoma MOPC-173 was induced by intraperitoneal injection of mineral oils into BALB/c mice.
Isotype IgG2a
Keywords Immunoglobulin Isotype Controls
Notes The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 μg/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody MOPC-173, generated against an undefined antigen, does not react specifically with mouse, rat and human samples, and hence all the background that could be observed when working with this antibody would be a result of general nonspecific interactions between an mouse IgG2a molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished.
Purification Purified by protein-A affinity chromatography
Purity > 95% (by SDS-PAGE)
Storage Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Substrate / Buffer Azide free phosphate buffered saline (PBS), approx. pH 7.4; 0.2 μm filter sterilized. Endotoxin level is less than 0.01 EU/μg of the protein, as determined by the LAL test.
Technical Specifications This mouse IgG2a monoclonal antibody (clone MOPC-173) reacts with an unknown epitope. It does not react with a variety of resting, activated, live, and fixed mouse, rat and human tissues.
Product Page Updated 2018-07-27T09:12:01.609Z

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