CD11b (mouse)

Article No AFC-4645-2
Product Type Antibodies Primary
Research Area Immunology
Application FC, IP, IHC-Fr, FA
Size 0.1 mg

Specifications

Country Availability all
BioSite Brand BioSite Flow
Clone M1/70
Clone Type monoclonal
Concentration 1 mg/ml
Description Rat Monoclonal to CD11b (mouse) / Mac-1 alpha
Supplier BioSite Flow
Immunogen B10 mouse spleen cells enriched for T cells_x000D_
Isotype IgG2b
Keywords CD Antigens & MHC (Veterinary), CD and Related Antigens & MHC (Rodent)
Notes CD11b (integrin alphaM subunit) is a 165-170 kDa type I transmembrane glycoprotein that non-covalently associates with integrin beta2 subunit (CD18); expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages. CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component._x000D_
Purification Purified by protein-G affinity chromatography
Purity > 95% (by SDS-PAGE)
Species Reactivity human, monkey, mouse, rabbit
Storage Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Substrate / Buffer Azide free phosphate buffered saline (PBS), approx. pH 7.4; 0.2 μm filter sterilized. Endotoxin level is less than 0.01 EU/μg of the protein, as determined by the LAL test.
Technical Specifications The rat monoclonal antibody M1/70 detects CD11b (integrin alphaM subunit), a type I transmembrane protein mainly expressed on monocytes/macrophages, granulocytes and NK-cells, which associates with CD18 to form Mac-1 integrin that plays important role in cell-cell interactions._x000D_
Product Page Updated 2018-07-27T09:12:01.609Z

Shipping info

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Species reactivity:

human, monkey, mouse, rabbit human monkey mouse rabbit

Suggested protocols:

Immunoprecipitation

Immunoprecipitation