Article No
AFC-5FIOQV-0.1
Anti-Ms CD3 Purified Low Endotoxin
Anti-Ms CD3 Purified Low Endotoxin
Antibodies Primary
Specifications
| Application | FC, IP, IHC, ICC |
| Article No | AFC-5FIOQV-0.1 |
| Biosite Brand | Biosite Flow |
| Country Availability | all |
| Clone | 145-2C11 |
| Clone Type | monoclonal |
| Concentration | 1 mg/ml |
| Conjugation | Unconjugated |
| Description | Anti-Ms CD3 Purified Low Endotoxin |
| Supplier | Nordic Biosite |
| Entrez Gene ID | 12501 |
| Format | Purified Low Endotoxin |
| Immunogen | Mouse BM10-37 cytotoxic T lymphocytes |
| Isotype | IgG k |
| Keywords | CD and Related Antigens & MHC (Rodent) |
| Notes | CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkynje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. |
| Alias Names | CD3E, T3E, TCRE |
| Previous Article No | AFC-4642-2, AFC-5FIOQV-0.1 |
| Product Type | Antibodies Primary |
| Protocol | Functional application: Induction of T cell activation, proliferation or apoptosis (depending on conditions), in vivo T cell depletion. Flow cytometry: Recommended dilution: 1-2 μg / ml (million cells). Immunoprecipitation: Recommended dilution: 1-2 μg / 100-500 μg protein in 1 ml of a cell lysate. |
| Purification | Purified by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Research area | Immunology |
| Size | 0.1 mg |
| Source / Host | mouse |
| Species Reactivity | mouse |
| Storage | Store at 2-8°C. Do not freeze. |
| Substrate / Buffer | Phosphate buffered saline (PBS), pH 7.4 |
| Technical Specifications | The Armenian hamster monoclonal antibody 145-2C11 reacts with an extracellular epitope of murine CD3 (epsilon subunit). This antibody is commonly used as a phenotypic marker for murine T cells. |
| UniProt Number | P22646 |
| Product Page Updated | 2024-01-03T12:18:20.318Z |
