Products ({{ products.totalElements }})
News & articles ({{ wpNewsAndArticles.total_posts }})
Blog entries ({{ wpBlogEntries.total_posts }})

{{ String(product.attributes.name) }} {{ String(product.attributes.productType) }} - NEW	{{ String(product.attributes.artNo) }}	{{ (String(product.attributes.sizeStr) != 'undefined') ? String(product.attributes.sizeStr) : '–' }}	Not available On Request {{ formatPrice(product.attributes.discountPrice.toString()) }} {{ formatPrice(product.attributes.price.toString()) }} {{ formatPrice(product.attributes.discountPrice.toString()) }} {{ formatPrice(product.attributes.price.toString()) }} Login for price

Full search results →

No products found

News {{ news.date \| formatDate }} #{{tag.name}}

No news & articles found

Full search results →

blog entry {{ blogEntry.date \| formatDate }}

No blog entries found

Full search results →

Anti-Hu CD3 Purified Low Endotoxin

Antibodies Primary

Make a customized order

Article No

AFC-UKTPR8-0.1

Species Reactivity

human

Size

0.1 mg

Clone

MEM-57

Source / Host

mouse

Application

FC, IP

Article No

AFC-UKTPR8-0.1

Species Reactivity

human

Size

0.1 mg

Clone

MEM-57

Source / Host

mouse

Application

FC, IP

Specifications
Suggested protocols
References

Specifications

Application	FC, IP
Article No	AFC-UKTPR8-0.1
Biosite Brand	Biosite Flow
Country Availability	all
Clone	MEM-57
Clone Type	monoclonal
Concentration	1 mg/ml
Conjugation	Unconjugated
Description	Anti-Hu CD3 Purified Low Endotoxin
Supplier	Nordic Biosite
Entrez Gene ID	916
Format	Purified Low Endotoxin
Immunogen	Human thymocytes and T lymphocytes.
Isotype	IgG2a k
Keywords	CD and Related Antigens (Human)
Notes	CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkynje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.
Alias Names	CD3E, T3E, TCRE
Previous Article No	AFC-4617-2, AFC-UKTPR8-0.1
Product Type	Antibodies Primary
Protocol	Immunoprecipitation: The antibody MEM-57 immunoprecipitates from a detergent lysate of surface-radioiodinated T cells a strong zone of about 22 kDa and a weak 28-kDa zone, which is typical pattern yielded by a reference antibody Leu-4 (SK7). Functional application: The antibody MEM-57 has strong mitogenic effect on peripheral T lymphocytes, it reacts strongly with gamma/delta T lymphocytes. Flow cytometry: Recommended dilution: 1-4 μg/ml; positive control: peripheral blood lymphocytes, JURKAT human leukemia T cell line.
Purification	Purified by protein-A affinity chromatography.
Purity	> 95% (by SDS-PAGE)
Research area	Immunology
Size	0.1 mg
Source / Host	mouse
Species Reactivity	human
Storage	Store at 2-8°C. Do not freeze.
Substrate / Buffer	Phosphate buffered saline (PBS), pH 7.4
Technical Specifications	The antibody MEM-57 reacts with an extracellular epitope on gamma-epsilon and delta-epsilon dimers of human CD3 complex, a part of a bigger multisubunit T cell receptor complex (CD3/TCR) expressed on peripheral blood T lymphocytes and mature thymocytes.
UniProt Number	P07766
Product Page Updated	2024-01-03T12:18:20.318Z

Suggested protocols

Flow Cytometry

Immunoprecipitation

References

*Kanderova V, Kuzilkova D, Stuchly J, Vaskova M, Brdicka T, Fiser K, Hrusak O, Lund-Johansen F, Kalina T: High-resolution Antibody Array Analysis of Childhood Acute Leukemia Cells. Mol Cell Proteomics. 2016 Apr;15(4):1246-61., URL: http://www.ncbi.nlm.nih.gov/pubmed/26785729 ,*Majer F, Vlaskova H, Krol L, Kalina T, Kubanek M, Stolnaya L, Dvorakova L, Elleder M, Sikora J: Danon disease: a focus on processing of the novel LAMP2 mutation and comments on the beneficial use of peripheral white blood cells in the diagnosis of LAMP2 deficiency. Gene. 2012 May 1;498(2):183-95., URL: https://pubmed.ncbi.nlm.nih.gov/22365987/,*Horejsi V. et al.: Monoclonal antibodies against human leucocyte antigens. II. Antibodies against CD45 (T200), CD3 (T3), CD43, CD10 (CALLA), transferrin receptor (T9), a novel broadly expressed 18-kDa antigen (MEM-43) and a novel antigen of restricted expression (MEM-74). Folia Biol. (Praha) 34, 23 (1988). , URL: https://www.ncbi.nlm.nih.gov/pubmed/2968928?dopt=A,*Hilgert I. et al.: Therapeutic in vivo use of the A1-CD3 monoclonal antibody. Transplantation 55, 435 (1993)., URL: https://www.ncbi.nlm.nih.gov/pubmed/8434399?dopt=A,*Leukocyte Typing IV., Knapp W. et al. (Eds.), Oxford University Press (1989); p. 293., URL: ,*Dave VP, Cao Z, Browne C, Alarcon B, Fernandez-Miguel G, Lafaille J, de la Hera A, Tonegawa S, Kappes DJ: CD3 delta deficiency arrests development of the alpha beta but not the gamma delta T cell lineage. EMBO J. 1997 Mar 17;16(6):1360-70., URL: https://www.ncbi.nlm.nih.gov/pubmed/9135151?itool=,*Panyi G, Bagdány M, Bodnár A, Vámosi G, Szentesi G, Jenei A, Mátyus L, Varga S, Waldmann TA, Gáspar R, Damjanovich S: Colocalization and nonrandom distribution of Kv1.3 potassium channels and CD3 molecules in the plasma membrane of human T lymphocytes. Proc Natl Acad Sci U S A. 2003 Mar 4;100(5):2592-7., URL: https://www.ncbi.nlm.nih.gov/pubmed/12604782?itool,*Drbal K, Moertelmaier M, Holzhauser C, Muhammad A, Fuertbauer E, Howorka S, Hinterberger M, Stockinger H, Schütz GJ: Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement. Int Immunol. 2007 May;19(5):675-84., URL: https://www.ncbi.nlm.nih.gov/pubmed/17446208?itool,*Brdicková N, Brdicka T, Angelisová P, Horváth O, Spicka J, Hilgert I, Paces J, Simeoni L, Kliche S, Merten C, Schraven B, Horejsí V: LIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling. J Exp Med. 2003 Nov 17;198(10):1453-62., URL: https://www.ncbi.nlm.nih.gov/pubmed/14610046?itool,*Soucek J, Chudomel V, Hrubá A, Lindnerová G: Induction of NK and LAK activities in human lymphocyte culture by a cytosol fraction from leukemic myeloblasts and by monoclonal antibody CD 3. Neoplasma. 1991;38(1):33-41., URL: https://www.ncbi.nlm.nih.gov/pubmed/2011208?itool=,*Soucek J, Hilgert I, Budová I, Lindnerová G: Augmentation of NK cell activity and proliferation in cultured lymphocytes of leukemic patients by monoclonal antibodies CD3 and interleukin-2. Neoplasma. 1994;41(2):75-81., URL: https://www.ncbi.nlm.nih.gov/pubmed/8208318?itool=,*Leukocyte Typing III., McMichael M.J. et al. (Eds.), Oxford University Press (1987); p.611., URL: ,,*Huang Y, Wange RL: T cell receptor signaling: beyond complex complexes. J Biol Chem. 2004 Jul 9;279(28):28827-30., URL: https://pubmed.ncbi.nlm.nih.gov/15084594/,*Alarcón B, Swamy M, van Santen HM, Schamel WW: T-cell antigen-receptor stoichiometry: pre-clustering for sensitivity. EMBO Rep. 2006 May;7(5):490-5., URL: https://pubmed.ncbi.nlm.nih.gov/16670682/,*Kuhns MS, Davis MM, Garcia KC: Deconstructing the form and function of the TCR/CD3 complex. Immunity. 2006 Feb;24(2):133-9., URL: https://pubmed.ncbi.nlm.nih.gov/16473826/,,

Shipping info

The delivery time for this item is approximately 8-16 business days. Read more