Article No
AFC-Z4T7Y2-0.1
Application | FC, IP, WB, IHC, ICC |
Article No | AFC-Z4T7Y2-0.1 |
Biosite Brand | Biosite Flow |
Country Availability | all |
Clone | P1H12 |
Clone Type | monoclonal |
Concentration | 1 mg/ml |
Conjugation | Unconjugated |
Description | Anti-Hu CD146 Purified |
Supplier | Nordic Biosite |
Entrez Gene ID | 4162 |
Format | Purified |
Immunogen | cultured human umbilical cells |
Isotype | IgG1 |
Keywords | CD Antigens & MHC (Veterinary), CD and Related Antigens & MHC (Rodent), CD and Related Antigens (Human) |
Notes | CD146, also known as MCAM (melanoma cell adhesion molecule) or MUC18, is a heavily glycosylated transmembrane glycoprotein with more than 50% of the mass from carbohydrates. It is expressed on epithelial and endothelial cells, fibroblasts, multipotent mesenchymal stromal cells, activated T cells and activated keratinocytes, and on some cancer cells, especially melanoma. The presence of CD146 on circulating blood cells has been confined to the activated T cells rather than circulating endothelial cells. CD146 mediates heterophilic cell adhesion and regulates monocyte transendothelial migration. |
Alias Names | MelCAM, MCAM, MUC18 |
Previous Article No | AFC-4604-2, AFC-Z4T7Y2-0.1 |
Product Type | Antibodies Primary |
Protocol | Western blotting: Nonreducing conditions. Flow cytometry: Recommended dilution: 1-4 µg/ml |
Purification | Purified by protein-A affinity chromatography. |
Purity | > 95% (by SDS-PAGE) |
Research Area | Immunology |
Size | 0.1 mg |
Source / Host | mouse |
Species Reactivity | rabbit, dog/canine, mouse, human |
Storage | Store at 2-8°C. Do not freeze. |
Substrate / Buffer | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Technical Specifications | The mouse monoclonal antibody P1H12 recognizes an extracellular epitope of CD146, a 118 kDa transmembrane glycoprotein expressed on epithelial and endothelial cells, fibroblasts, multipotent mesenchymal stromal cells, melanoma cells, activated T cells and activated keratinocytes. |
UniProt Number | P43121 |
Product Page Updated | 2024-01-03T12:18:20.318Z |